氯乙烯作业工人的染色体畸变和姊妹染色单体交换

1978年对北京地区某聚氯乙烯工厂的14名工人和14名对照者的外周血淋巴细胞作了染色体畸变频率和姊妹染色单体交换数目的检查。 两种细胞遗传学技术——常规技术和SCE技术对长期接触环境中高浓度氯乙烯的工人的染色体的诱变效应都是灵敏的。 实验监测结果表明工人的染色体断裂频率和姊妹染色单体交换的数目都显著地高于对照组。14名工人的平均染色体断裂是16.5%,对照组是2.57%;12名工人的每个细胞的平均姊妹染色单体交换数目是8.27±0.45,对照组是4.13±0.11。

FREQUENCY OF CHROMOSOMAL ABERRATIONAND SISTER CHROMATID EXCHANGES FORVINYL CHLORIDE-EXPOSED WORKERS

In 1978, examinations of chromosome aberration and SCEs in cultured lymphocytes were performed on 14 workers from a PVC plant in Beijing in contrast with same number of controls.Recently, a new BrdU-Giemsa technique was developed for detecting SCEs in the present study. This BrdU-Giemsa technique was called "BAUG" technique (BrdU Incorporation, AO prestaining, UV-irradiated, and Giemsa staining). It gives a differential staining with extremely high-quality (see Fig. 1).The two cytogenetic techniques, routine and SCE, are equally suitable for testing high-dose vinyl chloride mutagenicity in vivo.Our positive results show that presence of chromosome damage is indicative of workers chronically exposed to vinyl chloride with significantly high frequencies of chromosome breakage and SCEs than the controls. The mean breakage frequency for 14 workers was 12.8%, 2.57% for the controls (see Table 1); and the mean SCEs for 12 workers was 8. 27 and 0.45 per cell, for 4.13 and 0.11 per cell for the controls (see table 2).The results are discussed with respect to relationship between chromosome damage scored as chromosome breakage frequency and as number of SCE per cell.