高浓度基质冲击后厌氧氨氧化菌活性恢复研究

试验研究了阶段式负荷提高法对高浓度基质抑制后反应器中厌氧氨氧化(Anammox)菌的活性恢复特性的影响,考察了活性恢复过程反应器各阶段的脱氮性能、胞外聚合物(EPS)组分及Anammox菌丰度的变化.结果表明,通过逐步提高氮负荷率,反应器中Anammox菌活性于57d内即可恢复至受损前状态,最终氮去除负荷达2.21 kg·m-3·d-1;Anammox泥中的EPS含量、紧密型EPS和松散型EPS中蛋白质与多糖的比例均呈现先降低后上升的趋势,当进水总氮(TN)为500 mg·L-1时,EPS含量及二者的比例均最低;Anammox菌丰度对废水中氮浓度的敏感度较高,在活性恢复过程中,TN浓度为700 mg·L-1时丰度最高,达2.4×1010copies·g-1VSS.

Study on the activity recovery of anammox bacteria influenced by the high concentration matrix

The activity recovery performance of the anaerobic ammonium oxidation (Anammox) bioreactor which had suffered from inhibition of high substrate was investigated; long term dynamic change, extracellular polymers (EPS) contents and abundance of anammox bacteria in anammox reaction were evaluated in this study with stagewise improving nitrogen loading rate. Results showed that the recovery experiment of anammox reactor was succeed and final nitrogen removal rate was approximately 2.21 kg·m^-3·d^-1 within 57 days, it is better than the nitrogen removal performance of the reactor before damaged; When influent concentration of total nitrogen was less than 500 mg·L^-1, the content of EPS, ratio of protein and polysaccharide (PN/PS) of both tightly bound (TB) EPS and loosely bound (LB) EPS were decreased gradually, while the concentration was 500~1000 mg·L^-1, the content of EPS, TB-PN/PS and LB-PN/PS were increased slightly; Abundance of anammox bacteria was changed in volatility with the peak of 2.4×10¹⁰ copies·g^-1 VSS at 700 mg·L^-1 as for the high sensitivity to nitrogen of wastewater, the highest.