| 降解剩余污泥中纤维素/半纤维素微生物富集培养实验研究 |
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| 引用本文： | 郝晓地,王吉敏,胡沅胜.降解剩余污泥中纤维素/半纤维素微生物富集培养实验研究[J].环境科学学报,2015,35(4):999-1005. |
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| 作者姓名： | 郝晓地 王吉敏 胡沅胜 |
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| 作者单位： | 1. 北京建筑大学市政工程系/北京应对气候变化研究和人才培养基地,北京,100044
2. 北京建筑大学市政工程系/北京应对气候变化研究和人才培养基地,北京100044;太原理工大学建筑设计研究院,太原030024 |
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| 基金项目： | "十二五"国家科技支撑计划(No.2012BAJ14B03) |
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| 摘 要： | 为促进剩余污泥中木质纤维素能源转化,首先需要探知污泥中是否存在可以降解纤维素/半纤维素的微生物.以木聚糖作为单一碳源分别从剩余污泥和厌氧消化污泥中富集培养可降解纤维素/半纤维素的微生物.实验结果显示,两种污泥源中均存在可降解纤维素/半纤维素的微生物,并可成功富集培养获得该类微生物.FISH与LIVE/DEAD实验证实,富集微生物为梭状芽孢杆菌属和芽孢杆菌属的混合物,并以梭状芽孢杆菌为主;富集微生物均具有很高活性.
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| 关 键 词： | 剩余污泥 木质纤维素 木聚糖 酶活性 荧光原位杂交(FISH) LIVE/DEAD染色 |
| 收稿时间： | 2014/5/26 0:00:00 |
| 修稿时间： | 2014/10/13 0:00:00 |
Experimental study on enriching culture of cellulosic /hemicellulosic microorganisms in excess sludge |
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| HAO Xiaodi,WANG Jimin and HU Yuansheng.Experimental study on enriching culture of cellulosic /hemicellulosic microorganisms in excess sludge[J].Acta Scientiae Circumstantiae,2015,35(4):999-1005. |
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| Authors: | HAO Xiaodi WANG Jimin and HU Yuansheng |
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| Institution: | Beijing University of Civil Engineering and Architecture/Beijing Climate Change Research and Education Centre, Beijing 100044;1. Beijing University of Civil Engineering and Architecture/Beijing Climate Change Research and Education Centre, Beijing 100044;2. The Institute of Architectural Design and Research of Taiyuan University of Technology, Taiyuan 030024;Beijing University of Civil Engineering and Architecture/Beijing Climate Change Research and Education Centre, Beijing 100044 |
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| Abstract: | To improve energy conversion of lignocelluloses contained in excess sludge, we need to understand if there are cellulosic /hemicellulosic microorganisms in excess sludge. Xylan was chosen as a sole carbon source for enriching culture of cellulosic /hemicellulosic microorganisms in both excess sludge and anaerobic digestion sludge. The experimental results revealed that there were cellulosic /hemicellulosic microorganisms in the two types of sludge and that they were successfully enriched. Both FISH and LIVE/DEAD experiments verified that the enriched microorganisms were a mixture of Clostridium and Bacillus and dominated by Clostridium. The enriched microorganisms had very high activity. |
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| Keywords: | excess sludge lignocelluloses xylan enzymatic activity fluorescence in-situ hybridization (FISH) LIVE/DEAD staining |
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