过氧化氢对铜绿微囊藻的损伤效应研究

采用室内培养的方法研究了不同浓度H2O2对铜绿微囊藻(Microcystic aeruginosa)藻细胞的损伤效应.结果表明,H2O2浓度越大,对藻细胞的毒害作用越大.24 h之后,铜绿微囊藻藻细胞数、叶绿素a、类胡萝卜素和蛋白质含量及总抗氧化能力(T-AOC)都快速下降,而MDA含量显著增加,同时培养基中的H2O2含量也迅速降低;随着处理时间的增加,毒性效应逐渐增强;72 h之后,藻细胞各指标值都降得很低,MDA含量也增加到最大,培养基中H2O2也逐渐被消耗和分解,藻细胞的损伤效应也达到最大.其中,H2O2浓度为50 mg·L-1时,能够有效的去除藻细胞,并且对藻细胞的生理指标及抗氧化能力都有很强的损伤效应.

Damaging effects of hydrogen peroxide on Microcystis aeruginosa

This study used indoor culture method to investigate the damaging effect of hydrogen peroxide (H₂O₂) on Microcystis aeruginosa. Results showed that the toxic effect increased with increasing H₂O₂ concentrations. The number of algal cells, the content of chlorophyll a, carotenoid and protein, and T-AOC of Microcystis aeruginosa reduced rapidly after 24 hours, while the content of MDA increased significantly. Meanwhile, the H₂O₂ content in culture medium also reduced rapidly. Toxic effect also increased gradually with the processing time. The value of each index reduced to a very low level after 72 hours, the content of MDA increased to the maximum, and the H₂O₂ was consumed and decomposed gradually, implying that a maximum damage effect of algal cells was achieved. At 50 mg · L^-1, H₂O₂ can effectively remove algal cells, and exert a strong damaging effect on the physiological indexes and anti-oxidizing capacity of algal.