兽药阿苯达唑对蚯蚓精子发生的显微和超显微结构的影响

选择不影响蚯蚓生长的染毒剂量(100mg·kg-1)和半数致死剂量(600mg·kg-1),检测兽药阿苯达唑对蚯蚓精子发生的显微和超显微结构的影响.结果表明,光镜下观察贮精囊时,100mg·kg-1的阿苯达唑对精细胞和精子发育没有影响,而600mg·kg-1时,精细胞发育明显受阻,且未见成熟的精子.电镜下观察贮精囊时,100mg·kg-1的阿苯达唑损伤了精子发生的超微结构,表现为初期精细胞核萎缩变形,微管排列紊乱,精细胞膜扩张;发育后期精子细胞的线粒体嵴和基质消失;成熟精子的糖原颗粒异常分布于核周围,鞭毛的电子密度和糖原颗粒增加.而阿苯达唑剂量为600mg·kg-1时,精细胞严重损伤,核膜消失,甚至染色质消失.可见,精子发生的超微结构变化可以为早期土壤污染预警提供理论基础.

Effect of albendazole on microstructure and ultrastructure during spermiogenesis in earthworms

The study was to investigate the effect of veterinary medicine albendazole on microstructure and ultrastructure during spermiogenesis in earthworms.Earthworms were exposed to albendazole at 0 mg·kg^-1 (control),100 mg·kg^-1 (no effect on earthworms growth) and 600 mg·kg^-1 (LD₅₀).Under optical microscopy,no changes in seminal vesicle were observed at 100 mg·kg^-1 compared to the control,while the spermatid development was inhibited at 600 mg·kg^-1,with no mature sperms being found.Electron microscopy revealed that ultrastructure during spermiogenesis changed at 100 mg·kg^-1.In the early stage of spermiogenesis,the deformed nuclear was observed at 100 mg·kg^-1,microtubule's arrangement was abnormal and outer membrane of spermatid was enlarged.In the late stage,the cristae and matrix content in mitochondria disappeared at 100 mg·kg^-1,and dense glycogen granules were located abnormally around nuclear.We also found out that electron dense and glycogen granules in flagellum piece increased at 100 mg·kg^-1.The spermatid was damaged seriously at 600 mg·kg^-1,characterized with nuclear membrane and chromatin disappearing.This study suggested that changes of ultrastructure during spermiogenesis can provide theoretical foundation to early pollution prediction of veterinary medicine residue in soil.