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| 邻苯二甲酸二(2-乙基己基)酯酶促降解性的研究 | |
| 引用本文： | 曾锋,康跃惠,傅家谟,盛国英,杨惠芳,张展霞.邻苯二甲酸二(2-乙基己基)酯酶促降解性的研究[J].环境科学学报,2001,21(1):13-17. |
| 作者姓名： | 曾锋 康跃惠 傅家谟 盛国英 杨惠芳 张展霞 |
| 作者单位： | 1. 中山大学化学与化学工程学院, 2. 中国科学院生态环境研究中心 3. 中国科学院广州地球化学研究所 4. 中国科学院微生物研究所 |
| 基金项目： | 国家自然科学重点基金项目 !(4 96 32 0 6 0 )，有机地球化学国家重点实验室科学基金! (OGL 990 1) |
| 摘 要： | 从处理石化厂废水的活性污泥中分离出一株邻苯二甲酸酯降解菌FS1（Pseudomonas fluorescens FS1),测定了P.fluorescens FS1的邻苯二甲酸酯降解酸对邻苯二甲酸二（2－乙基已基）酯（DEHP）的降解特性。P.fluorescens FS1细胞中的颗粒部分、溶液部分对DEHP都具有降解作用，初步认为P.fluorescens FS1降解酶属胸内酶。P.fluorescens FS1降解酶对DEHP的最适酸度为pH6.5-8.0，温度为25－35℃，米氏常数Km为190.48nmol/mL。在好氧条件下，DEHP与P.fluorescens FS1降解酯酶作用，形成邻苯二甲酸单酯和邻苯二甲酸后，可进一步降解成苯甲酸、对羟基苯甲酸，最终转化为CO2与H2O。 |
| 关 键 词： | 邻苯二甲酸酯 乙基已基 微生物 酶促降解 生物降解 有机污染物 |
| 文章编号： | 0253-2468(2001)-01-0013-05 |
| 收稿时间： | 1999/12/17 0:00:00 |
| 修稿时间： | 1999年12月17 |
Study of enzymatic degradability of di(2-ethylhexyl)phthalate |
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| ZENG Feng,KANG Yuehui,FU Jiamo,SHENG Guoying,YANG Huifang and Zhang Zhanxia.Study of enzymatic degradability of di(2-ethylhexyl)phthalate[J].Acta Scientiae Circumstantiae,2001,21(1):13-17. | |
| Authors: | ZENG Feng KANG Yuehui FU Jiamo SHENG Guoying YANG Huifang and Zhang Zhanxia |
| Institution: | School of Chemistry and Chemical Engineering, Zhongshan University, Guangdong 510275,Research Center for Eco-Enviromental Science, Chinese Academy of,State Key Laboratory of Organic Geochemistry, Guangdong Key Laboratory of Environment Protection and Resources Utilization, Guangzhou Institute of Geochemistry, Chinese Academy Sciences,State Key Laboratory of Organic Geochemistry, Guangdong Key Laboratory of Environment Protection and Resources Utilization, Guangzhou Institute of Geochemistry, Chinese Academy Sciences,Institute of Microbiology, Chinese Academy of Sciences and School of Chemistry and Chemical Engineering, Zhongshan University, Guangdong 510275 |
| Abstract: | Degradative characteristics of di(2-ethylhexyl)phthalate were determined by the crude enzyme extracted from Pseudomonas fluorescens FS1 which was isolateed from the activated sludge at a petrochemical factory. The results showed that the PAEs-degrad-ing enzyme, mainly locatized in soluble part of cytoplasm, is an intracllular enzyme. The optium temperature and pH of the PAEa-de-grading enzyme activity to di(2-ethylhexyl)phthalate are 20—35℃ and 6.5—8.0, respectively . The Michaelis-mentn' s constant (Km) of di(2-ethyl hexyl)phthalate enzymatic degradation are about 190.48 nmol/mL at 30℃. The enzymatic metabolism of di ((2-ethyl hexyl) ) phthalate under aerobic conditions begins by PAEs-degrading enzyme to monoester, further enzymatic degradation of monoester proceeds via phthalic acid by benzoate, 4-hydroxy benzoic acid, and then to CO2 and H2O. |
| Keywords: | di(2\|ethylhexyl)phthalate microorganism enzymatic degradation metabolic pathd |
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