| 奇异变形杆菌TJ-1产絮凝剂的培养基优化研究 |
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| 引用本文： | 杨阿明,夏四清,王学江,张志强.奇异变形杆菌TJ-1产絮凝剂的培养基优化研究[J].环境科学学报,2007,27(12):1988-1993. |
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| 作者姓名： | 杨阿明 夏四清 王学江 张志强 |
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| 作者单位： | 同济大学环境科学与工程学院,污染控制及资源化国家重点实验室,上海,200092 |
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| 基金项目： | 高等学校博士学科点专项科研基金
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教育部新世纪优秀人才支持计划 |
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| 摘 要： | 从活性污泥中分离出1株新型高效产絮凝剂菌TJ-1,经16S rDNA相似性分析鉴定为奇异变形杆菌(Proteus mirabilis),这是首次发现奇异变形杆菌能产微生物絮凝剂,对其进行了产絮凝剂培养条件优化研究.结果表明,TJ-1菌产絮凝剂的最佳培养基成分为:葡萄糖10g,蛋白胨1g,MgS040.3g,KH2PO42g,K2HP04 5g,去离子水1L,pH=7.0.絮凝实验结果表明,该菌在最佳培养条件下所产絮凝剂对高岭土悬液的絮凝率达到92%.
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| 关 键 词： | 奇异变形杆菌 生物絮凝剂 培养基 奇异变形杆菌 微生物絮凝剂 培养基成分 优化研究 Proteus culture conditions optimal 絮凝率 高岭土悬液 絮凝实验 去离子水 蛋白胨 葡萄糖 最佳 结果 培养条件 发现 相似性分析 rDNA 新型高效 |
| 文章编号： | 0253-2468（2007）12-1988-06 |
| 收稿时间： | 2006-11-30 |
| 修稿时间： | 2007-04-26 |
Study on the optimal flocculant-producing culture conditions of Proteus Mirabilis TJ-1 |
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| YANG Aming,XIA Siqing,WANG Xuejiang and ZHANG Zhiqiang.Study on the optimal flocculant-producing culture conditions of Proteus Mirabilis TJ-1[J].Acta Scientiae Circumstantiae,2007,27(12):1988-1993. |
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| Authors: | YANG Aming XIA Siqing WANG Xuejiang and ZHANG Zhiqiang |
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| Institution: | College of Environmental Science and Engineering, State Key Laboratory of Pollution Control and Resource Reuse, Tongji University, Shanghai 200092,College of Environmental Science and Engineering, State Key Laboratory of Pollution Control and Resource Reuse, Tongji University, Shanghai 200092,College of Environmental Science and Engineering, State Key Laboratory of Pollution Control and Resource Reuse, Tongji University, Shanghai 200092 and College of Environmental Science and Engineering, State Key Laboratory of Pollution Control and Resource Reuse, Tongji University, Shanghai 200092 |
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| Abstract: | A new kind of high flocculant-producing strain TJ-1 was obtained from activated sludge.It was identified as Proteus mirabilis by analysis of 16S rDNA similarity.This is the first time to identify Proteus mirabilis strain is able to produce microbial flocculant.The flocculant-producing cultivation conditions were studied and the culture media which optimized production of bioflocculant by strain TJ-1 were:glucose 10 g,peptone 1 g,MgSO4 0.3 g,KH2PO4 2 g,and K2HPO4 5 g in 1 L H2O at pH 7.The flocculation test results showed that the flocculating activity towards a kaolin suspension reached 92% when the bioflocculant was produced at the optimal culture conditions. |
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| Keywords: | Proteus mirabilis bioflocculant culture medium |
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