不同群体形态蓝藻的气囊与光的相互作用研究

从气囊破裂前后藻液的浊度和吸收光谱的变化,以及气囊压力破裂曲线的变化对气囊与光的相互作用进行了表征.结果表明,气囊在向前方向上对光的散射作用能增强光的透过性;气囊对细胞体积的增大作用有利于蓝藻对光的吸收,这种作用在群体型蓝藻和丝状蓝藻中更为明显.波长较长的红光较波长较短的蓝光具有更强的透过性,这种作用在群体和丝状蓝藻中也很明显.在群体和丝状蓝藻中,由于光的多次散射,采用光学的方法测定气囊的相对含量可能产生很大的误差,而由于群体蓝藻易于富集,因而可用压力毛细管的方法进行气囊绝对含量的测定.

Interactions of light with gas vesicles in cyanobacteria of different colonial morphology

Many kinds of planktonic cyanobacteria possess gas vesicles, which interacts with light in a number of ways. To investigate the interactions, absorption spectra and turbidity of cyanobacteria suspension with intact and collapsed gas vesicles was analyzed, and absolute gas vesicle volume was determined. Light transmission in algal suspension was enhanced due to light scattering of gas vesicles in forward direction, on the other hand, increased cell volume by gas vesicles favored light absorption by intact cells and the effect was more evident in colonial and filamentous cyanobacteria. Light with longer wavelength (e.g. red light) had higher transmissionin cyanobacteria suspension than light with shorter wavelength, and similarly the effect was more evident in colonial and filamentous cyanobacteria. Due to the multiple scattering of light in colonial and filamentous cyanobacteria, measurement of relative gas vesicles volume by optical methods may result in great errors, instead of that capillary pressure tube method is suitable for the measurement of absolute volume of gas vesicles because colonial and filamentous cyanobacteria can be easily concentrated.