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能源植物修复土壤镉污染过程中细菌群落分析
引用本文:丁传雨,郑远,任学敏,陈兆进.能源植物修复土壤镉污染过程中细菌群落分析[J].环境科学学报,2016,36(8):3009-3016.
作者姓名:丁传雨  郑远  任学敏  陈兆进
作者单位:南阳师范学院农业工程学院, 南阳 473061,南阳师范学院农业工程学院, 南阳 473061,南阳师范学院农业工程学院, 南阳 473061,南阳师范学院农业工程学院, 南阳 473061
基金项目:南阳师范学院高层次人才科研启动费资助项目(No.ZX2014069,ZX2014066);河南省教育厅高等学校重点科研项目(No.16A210012,14A180015)
摘    要:选取油脂类能源植物大豆和碳水化合物类能源植物玉米,采用高通量测序方法研究大豆、玉米修复Cd污染土壤过程中根际土壤细菌群落组成.结果表明,100 mg·kg-1Cd的添加会抑制玉米、大豆生长,其中,大豆生物量降低比玉米高.不同组织中根部Cd积累量最高,转移系数TF分别为0.56(玉米)和0.14(大豆).基于Mi Seq的群落分析表明,大豆、玉米根际土壤细菌主要包括Proteobacteria(变形菌门)、Acidobacteria(酸杆菌门)、Gemmatimonadetes(芽单胞菌门)、Actinobacteria(放线菌门)、Bacteroidetes(拟杆菌门)等33个门.细菌群落的PCo A和UPGMA分析表明,Cd的添加和能源植物种植均能对细菌群落结构产生影响,其中,Cd的添加影响最大.Gemmatimonas、Flavisolibacter、Flexibacter、Ramlibacter、Ohtaekwangia、Flavitalea等细菌在Cd胁迫条件或大豆、玉米种植条件下相对丰度有所变化,分析其可能在大豆、玉米耐受Cd污染中起作用.

关 键 词:镉污染  植物修复  能源植物  高通量测序  细菌群落
收稿时间:2015/10/9 0:00:00
修稿时间:2015/11/2 0:00:00

Changes in bacterial community composition during the remediation of Cd-contaminated soils of bioenergy crops
DING Chuanyu,ZHENG Yuan,REN Xueming and CHEN Zhaojin.Changes in bacterial community composition during the remediation of Cd-contaminated soils of bioenergy crops[J].Acta Scientiae Circumstantiae,2016,36(8):3009-3016.
Authors:DING Chuanyu  ZHENG Yuan  REN Xueming and CHEN Zhaojin
Institution:College of Agricultural Engineering, Nanyang Normal University, Nanyang 473061,College of Agricultural Engineering, Nanyang Normal University, Nanyang 473061,College of Agricultural Engineering, Nanyang Normal University, Nanyang 473061 and College of Agricultural Engineering, Nanyang Normal University, Nanyang 473061
Abstract:This study investigated the bacterial community composition in rhizosphere soils of 2 bioenergy crops (high starch crop-maize and oilseed crop-soybean) using high-throughput sequencing in the phytoremediation of Cd-contaminated soils. The result showed significantly lower dry weights of maize and soybean plants when 100 mg·kg-1 Cd was added to the soil, compared to the controls without Cd contamination. The decrease in the dry weights in the roots, stems, and leaves of the maize plants was 24.53%, 35.34% and 14.59%, respectively, and that of soybean plants was 63.67%, 75.75% and 67.70%, respectively. Cd concentration in the roots of maize and soybean plants was significantly higher than that in the stems and leaves. The translocation factor of maize and soybean plants was 0.56 and 0.14, respectively. The diversity of rhizosphere bacteria from maize and soybean plants was evaluated using high-throughput sequencing in the phytoremediation of the Cd-contaminated soils of these plants. Using Illumina MiSeq sequencing, a set of more than 32769 effective sequence tags were obtained for each sample. Tags with 97% similarity were then grouped into OTUs using Mothur and Qiime, and 1563 OTUs were obtained for each sample. Phylogenetic analysis based on 16S rDNA sequences showed that the rhizosphere bacteria could be divided into 33 major phylogenetic groups by phyla. The dominant phylogenetic groups included Proteobacteria, Acidobacteria, Gemmatimonadetes, Actinobacteria, and Bacteroidetes. Based on the principal coordinate analysis (PCoA) and UPGMA, the bacterial community composition was found to be primarily affected by Cd addition and bioenergy cropping. At the genus level, the relative abundance of Gemmatimonas species significantly increased with soybean planting, and it decreased with Cd addition and maize cropping. The relative abundance of Gemmatimonas, Flavisolibacter, Flexibacter, Ramlibacter, Ohtaekwangia, and Flavitalea species were affected by Cd addition and bioenergy cropping. The results of this study offer theoretical and experimental bases for the application of plant growth promoting bacterium-enhanced phytoremediation.
Keywords:Cd contamination  phytoremediation  bioenergy crop  high-throughput sequencing  bacterial community
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