酶法原位制备过氧乙酸对活性艳蓝KN-R氧化脱色的工艺

利用过水解酶催化合成过氧乙酸,过氧乙酸原位氧化活性艳蓝KN-R脱色.在单因子试验的基础上,通过正交优化试验确定活性艳蓝KN-R的最佳脱色条件为:在5 m L反应体系中,最适p H 5.0,加酶量为20 U·反应-1,乙酸乙酯对过氧化氢的摩尔比率为40∶1和活性艳蓝KNR的浓度为80 mg·L-1.在此条件下反应6 h后,活性艳蓝KN-R的脱色率为81.11%,24 h后的脱色率为91.96%.在50倍放大试验中,该工艺24小时的脱色率为84.55%.

Decolorization of remazol brilliant blue KN-R by the in-situ enzymatically generated peracetic acid

A novel biological and chemical coupled treatment technology for decolorization of Remazol Brilliant Blue R was developed in this research work. Peracetic acid was first enzymatically prepared with perhydrolysis reaction and then applied to in situ oxidize Remazol brilliant blue KN-R (RBBR), one of the widely used dyes in the printing and dying industry. Based on the results of the single factor test, the optimal decolorization conditions for RBBR was then obtained via orthogonal experiment. The optimized physical and chemical factors for the oxidative decolorization of RBBR were found to be: 20 U per reaction enzyme dosage, the molar ratio of ethyl acetate to hydrogen peroxide at 40 : 1, 80 mg · L^-1 RBBR concentration, and intial pH5.0 in the 5 mL decolorization system. The maximal decolorization of RBBR reached 81.11% for 6 h and 91.96% for 24 h in the 5 mL decolorization system, respectively. However, the maximal decolorization decreased to 84.55% for 24 h in the 250 mL decolorization system.