| 小麦中T-2毒素酶联免疫吸附测定法(ELISA) |
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| 引用本文： | 阳传和,罗雪云,计融,孙艺.小麦中T-2毒素酶联免疫吸附测定法(ELISA)[J].环境科学学报,1992,12(3):372-376. |
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| 作者姓名： | 阳传和 罗雪云 计融 孙艺 |
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| 作者单位： | 卫生部食品卫生监督检验所 北京100021
(阳传和，罗雪云，计融)，卫生部食品卫生监督检验所 北京100021(孙艺) |
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| 基金项目： | 国家“七五”攻关课题《食品卫生检测技术——食品中有害物质分析》内容之一 |
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| 摘 要： | T-2毒素是某些植物致病菌的次生代谢产物,尤其是由镰刀菌(Fusarium sp)引致的麦类、玉米等作物的赤霉病,菌体在一定条件下能产生大量的该类毒素.T-2毒素对人畜的毒性很大,同时,还与某些癌症的发病率有密切的关系.T-2毒素的检测方法,常用的有薄层层析法,气相、液相色谱法以及气-质联用等,这些方法,有的精确度
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| 关 键 词： | 小麦 T－2毒素 酶联免疫吸附 测定法 |
| 收稿时间： | 1991/3/10 0:00:00 |
ENZYME-LINKED IMMUNOSORBENT ASSAY FOR T-2 TOXIN IN WHEAT |
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| Yang Chuanhe,Luo Xueyun,Ji Rong and Sun Yi.ENZYME-LINKED IMMUNOSORBENT ASSAY FOR T-2 TOXIN IN WHEAT[J].Acta Scientiae Circumstantiae,1992,12(3):372-376. |
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| Authors: | Yang Chuanhe Luo Xueyun Ji Rong and Sun Yi |
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| Institution: | Institute of Food Safety Control and Inspection, Ministry of Public Health, Beijing 100021,Institute of Food Safety Control and Inspection, Ministry of Public Health, Beijing 100021,Institute of Food Safety Control and Inspection, Ministry of Public Health, Beijing 100021 and Institute of Food Safety Control and Inspection, Ministry of Public Health, Beijing 100021 |
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| Abstract: | An indirect enzyme-linked immunosorbent assay method has been developed for determination of T-2 toxin in wheat. The wheat flour sample was extra cted with chloriform-ehanol(8:2) and the extract was washed with hexane and passed aluminum oxide/charcoal cleanup columns. The extract was then evaporated to remove the organic solvent, diluted with 20% methanol with 0.1 mol sodium phosphate buffer (pH 7.4), and used for ELSA. The minimal, detection level for T-2 was Ing/m1(0. ing/assay).The mean recoveries and CV% for wheat spiked with 1-500ng/g of T-2 toxin were 80.4-110.7% and 4.7-19.2%, respectively. Analysis of 10 naturally conta minated samples for T-2 showed positive results. The concentrations found was butween 8 and 126.4ng/g. |
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| Keywords: | wheat T-2 toxin enzyme-linked immunosorbent assay |
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