沙尘暴细颗粒物对人外周血淋巴细胞微核形成的影响

用胞质阻断微核试验法比较研究了内蒙古包头市和甘肃武威市沙尘暴和正常天气细颗粒物(PM2.5)及其无机与有机提取物对人外周血淋巴细胞微核形成的影响.结果表明本试验条件下,沙尘暴和正常天气PM2.5及其有机提取物均使淋巴细胞微核率(micronuclei frequency,MNF)显著增高,核分裂指数(nuclear division index,NDI)显著下降(p＜0.01),且存在剂量-效应关系,而无机提取物虽有剂量-效应关系,但各处理浓度之间及其与对照相比差异均不显著(p＞0.05);相同处理浓度下,除工业城市包头正常天气的PM2.5及其有机提取物处理的微核率显著高于沙尘(p＜0.01)外,沙尘暴和正常天气样品处理结果无显著差异(p＞0.05);城市之间相比,只有正常天气PM2.5及其有机提取物处理的微核率包头显著高于武威(p＜0.01),而沙尘暴PM2.5及其提取物的所有处理的微核率无城市间差异(p＞0.05).

Effects of dust storm fine particles (PM2.5) on micronuclei formation of human blood lymphocytes

The effects of dust storm and normal ambient air fine particles (PM2.5) and their organic and inorganic extracts from Baotou city and Wuwei city on micronuclei formation of human blood lymphocytes were invested using cytochalasin B blocked test. The results showed that dust storm and normal ambient air PM2.5 and their organic extracts had marked genetic toxicity on lymphocytes. With the increase of treatment concentrations the micronuclei frequency (MNF) increased and the nuclear division index (NDI) values declined in a dose-response manner(p<0.01). Although the inorganic extracts showed dose-response manner but there was no marked difference among the treatments(p>0.05). In the same concentrations, the treatments of dust storm samples and normal samples were not significant different (p>0.05) except the treatments of normal ambient air PM2.5 from Baotou city and its organic extracts were significant higher than those of dust storm(p<0.01). The treatments of normal ambient air PM2.5 from Baotou city and its organic extracts were significant higher than those of Wuwei city, but the treatments of dust storm PM2.5 and its extracts were not significant different between the cities.