模拟N、P污水对水稻幼苗过氧化物酶和超氧化物歧化酶的影响

以水稻为材料，研究了不同浓度的模拟Ｎ、Ｐ污水对幼苗过氧化物酶（ＰＯＤ，ＥＣ１．１１．１．７）和超氧化物歧化酶（ＳＯｏＤ，ＥＣ１．１５．１．１）及其同工酶等的影响，结果表明：在总氮浓度（Ｎｒ）为０─９０ｍｇ／Ｌ和总磷浓度（Ｐ＿Ｔ）为０─９ｍｇ／Ｌ范围内，ＰＯＤ与Ｎ、Ｐ浓度呈正相关，Ｎ＿Ｔ超过９０ｍｇ／Ｌ和Ｐ＿Ｔ超过９ｍｇ／Ｌ这一阈值后呈下降趋势，ＮＴ在０─１２０ｍｇ／Ｌ和Ｐ＿Ｔ在０─１２ｍｇ／Ｌ范围内，ＳＯＤ，丙二醛（ＭＤＡ）与Ｎ、Ｐ浓度呈显著正相关；Ｎ、Ｐ能诱导产生新的同工酶带，苗高、可溶性蛋白分别与Ｎ、Ｐ浓度呈显著负相关．

EFFECTS OF MIMIC WASTEWATER CONTAINING N,P ON PEROXIDASE AND SUPEROXIDE DISMUTASE IN RICE SEEDLINGS

EHects of mimic wastewater on activities andisoenzyme of peroxdase(EC1.11.1.7POD)and superoxide dismutase(SOD, EC 1.15.1.1)were studied at various concentra-tions of nitrogen and phosphorus, The results showed that when N_T<90 mg/L and P_T<9mg/L, there were significant positive correlations between POD activity and the concentra-tion of N and P,while POD activity declined when N_T > 9 0 mg/L and P_T > 9 mg/L. SODactivity and content of malondialdehyde(MDA)were positively correlated to the concentra-tion of N and P significant.In addition,effects of N and P on heights of rice seedlings andcontents of soluble protein in their leaves were studied.The results indicated that heightsnegatively correlated to the concentration of N and P,and so did the contents of soluble pro- tein.