| 嗜根寡养单胞菌DSM14405T对高浓度Cr(Ⅵ)的抗性及其还原特性分析 |
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| 引用本文： | 吴诗敏,高婕,刘颖,白志辉,吴尚华,庄国强,庄绪亮.嗜根寡养单胞菌DSM14405T对高浓度Cr(Ⅵ)的抗性及其还原特性分析[J].环境科学学报,2019,39(11):3755-3763. |
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| 作者姓名： | 吴诗敏 高婕 刘颖 白志辉 吴尚华 庄国强 庄绪亮 |
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| 作者单位： | 中国科学院生态环境研究中心环境生物技术重点实验室,北京100085;中国科学院大学资源与环境学院,北京100049;中国科学院生态环境研究中心环境生物技术重点实验室,北京100085;中国科学技术大学生命科学学院,合肥230026 |
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| 基金项目： | 国家自然科学基金（No.31670507，41501250）；中国科学院重点部署项目（No.ZDRW-ZS-2016-5）；国家重点研发计划（No.2016YFC0500401） |
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| 摘 要： | 为了寻找植物-微生物联合治理Cr（VI）污染的合适微生物，分析了一株植物根际促生菌——嗜根寡养单胞菌DSM 14405T（Stenotrophomonas rhizophila DSM 14405T）对不同浓度Cr（VI）的还原能力、Cr（VI）还原最适培养条件、对连续投加Cr（VI）的还原能力及高浓度Cr（VI）处理不同时间的转录组变化.结果表明，嗜根寡养单胞菌DSM 14405T在24 h内对10、50、200和500 mg·L-1 Cr（VI）的还原率分别为100%、92.4%、26.2%和16.5%，且在30℃、pH=7.5、转速180 r·min-1条件下培养能最经济、高效地还原Cr（VI）.该菌能在64 h内完全还原2次所投加的50 mg·L-1的Cr（VI），第3次投加后仅能还原55.2%左右.比较转录组学分析可得，200 mg·L-1Cr（VI）作用下，嗜根寡养单胞菌DSM 14405T细胞表现出显著的抗性.具体表现为：大幅度上调胞内消除有毒自由基的相关基因、DNA修复相关基因以防止DNA因自由基受损，同时下调细胞组分及蛋白代谢等与细胞抗性相关性低的通路.并且，随着Cr（VI）的还原和细胞对环境的适应，Cr（VI）抗性相关基因及通路的调节在长时暴露中明显少于短时暴露.本研究为Cr（VI）污染的原位植物-微生物联合治理提供了可选菌株和理论依据.
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| 关 键 词： | 嗜根寡养单胞菌DSM 14405T Cr(Ⅵ) 生物还原 细胞抗性 |
| 收稿时间： | 2019/2/26 0:00:00 |
| 修稿时间： | 2019/3/18 0:00:00 |
The resistance of Stenotrophomonas rhizophila DSM 14405T to high concentration of Cr(VI) and its reduction traits |
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| WU Shimin,GAO Jie,LIU Ying,BAI Zhihui,WU Shanghu,ZHUANG Guoqiang and ZHUANG Xuliang.The resistance of Stenotrophomonas rhizophila DSM 14405T to high concentration of Cr(VI) and its reduction traits[J].Acta Scientiae Circumstantiae,2019,39(11):3755-3763. |
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| Authors: | WU Shimin GAO Jie LIU Ying BAI Zhihui WU Shanghu ZHUANG Guoqiang and ZHUANG Xuliang |
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| Institution: | 1. CAS Key Laboratory of Environmental Biotechnology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085;2. College of Resources and Environment, University of Chinese Academy of Sciences, Beijing 100049,1. CAS Key Laboratory of Environmental Biotechnology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085;2. College of Resources and Environment, University of Chinese Academy of Sciences, Beijing 100049,1. CAS Key Laboratory of Environmental Biotechnology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085;2. School of Life Sciences, University of Science and Technology of China, Hefei 230026,1. CAS Key Laboratory of Environmental Biotechnology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085;2. College of Resources and Environment, University of Chinese Academy of Sciences, Beijing 100049,1. CAS Key Laboratory of Environmental Biotechnology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085;2. College of Resources and Environment, University of Chinese Academy of Sciences, Beijing 100049,1. CAS Key Laboratory of Environmental Biotechnology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085;2. College of Resources and Environment, University of Chinese Academy of Sciences, Beijing 100049 and 1. CAS Key Laboratory of Environmental Biotechnology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085;2. College of Resources and Environment, University of Chinese Academy of Sciences, Beijing 100049 |
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