| 诺卡氏菌株C-14-1中腈水解酶基因的鉴定、测序及分析 |
| |
| 引用本文： | 方萍,徐德强,张亚雷,曹薇寰,赵建夫,朱颖旻,覃重军.诺卡氏菌株C-14-1中腈水解酶基因的鉴定、测序及分析[J].环境科学学报,2005,25(10):1414-1417. |
| |
| 作者姓名： | 方萍 徐德强 张亚雷 曹薇寰 赵建夫 朱颖旻 覃重军 |
| |
| 作者单位： | 同济大学环境科学与工程学院,污染控制与资源化国家重点实验室,生物技术中心,上海,200092;中国科学院上海生命科学院植物生理生态研究所,上海,200032;复旦大学生命科学学院,上海,200433;同济大学环境科学与工程学院,污染控制与资源化国家重点实验室,生物技术中心,上海,200092;中国科学院上海生命科学院植物生理生态研究所,上海,200032 |
| |
| 基金项目： | 国家杰出青年基金项目（No.30325003）;国家自然科学基金项目（No.50008011）. |
| |
| 摘 要： | 从腈纶废水中分离到高效降解多种污染物的诺卡氏菌株C-14—1，并对该菌中腈水解酶的基因进行鉴定和测序．利用红球菌中腈水解酶氨基酸保守区设计核苷酸引物，以菌株C-14—1的总DNA为模板，PCR扩增发现一条预期大小的DNA带．Southern杂交显示基因组中存在一个腈水解酶基因．进一步构建基因文库和菌落原位杂交，克隆到一个约4．5kb的DNA片段．DNA序列测定和分析表明，该DNA片段携带长度为1143bp的腈水解酶基因．比对分析表明，该基因与国际上发表的红球菌和诺卡氏菌中的腈水解酶基因高度相似．
|
| 关 键 词： | 诺卡氏菌 腈水解酶基因 DNA序列测定 |
| 文章编号： | 0253-2468(2005)10-1414-04 |
| 收稿时间： | 06 1 2005 12:00AM |
| 修稿时间： | 07 29 2005 12:00AM |
Identification, sequencing and analysis of the Nitrilase Gene from Nocardia sp. C-14-1 |
| |
| FANG Ping,XU Deqiang,ZHANG Yalei,CAO Weihuan,ZHAO Jianfu,ZHU Yingmin and QIN Zhongjun.Identification, sequencing and analysis of the Nitrilase Gene from Nocardia sp. C-14-1[J].Acta Scientiae Circumstantiae,2005,25(10):1414-1417. |
| |
| Authors: | FANG Ping XU Deqiang ZHANG Yalei CAO Weihuan ZHAO Jianfu ZHU Yingmin and QIN Zhongjun |
| |
| Institution: | 1. College of Environmental Sciences & Engineering, National Key laboratory of Pollution Control & Resources Reuse, Tongji University, Shanghai 200092; 2. Shanghai Institute of Plant Physiology and Ecology, Shanghai Institute of Biological Science,College of Biological Science, Fudan University, Shanghai 200433,College of Environmental Sciences & Engineering, National Key laboratory of Pollution Control & Resources Reuse, Tongji University, Shanghai 200092,College of Environmental Sciences & Engineering, National Key laboratory of Pollution Control & Resources Reuse, Tongji University, Shanghai 200092,College of Environmental Sciences & Engineering, National Key laboratory of Pollution Control & Resources Reuse, Tongji University, Shanghai 200092,Shanghai Institute of Plant Physiology and Ecology, Shanghai Institute of Biological Sciences, Chinese Academy of Sciences, Shanghai 200032 and Shanghai Institute of Plant Physiology and Ecology, Shanghai Institute of Biological Sciences, Chinese Academy of Sciences, Shanghai 200032 |
| |
| Abstract: | An efficient degrader Nocardia sp. C-14-1 was isolated from acrylic fiber wastewater. The identification, cloning and sequencing of a gene encoding mtrilase from strain C-14-1 was introduced. Oligo-nucleotides were designed according to the conserved regions of nitrilnse genes from Rhodococcus, and an expected size of DNA band was detected after PCR amplification and electrophoresis. Southern blotting showed that there was a single nitrilase gene in the genome of C-14-1. A genome library was constructed and colony hybridization carried out, and a plasmid containing c. 4.5 kb DNA selected. DNA sequencing and analysis showed that there was a fragment of 1 143 bp DNA sequence encoded the nitrilase, which has highly significant similarity to the known nitrilase genes from Rhodococcus and Nocardia. |
| |
| Keywords: | Nocard/a gene encoding nitrilase DNA sequence |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
| 点击此处可从《环境科学学报》浏览原始摘要信息 |
| 点击此处可从《环境科学学报》下载免费的PDF全文 |
|
