低剂量长期铅暴露对雄性黑斑蛙精巢组织关键酶活性的影响

在实验条件下,将健康的性成熟雄性黑斑蛙暴露于0.1、0.2、0.4、0.8和1.6mg·L-1的Pb2+溶液中30d,以Ca2+-ATP酶、Na+-K+-ATP酶、Ca2+-Mg2+-ATP酶、β-葡萄糖醛酸苷酶(β-DG)、乳酸脱氢酶(LDH)和酸性磷酸酶(ACP)活性为指标,进行了长期铅暴露对雄性黑斑蛙生殖毒性的研究.结果表明:随着铅染毒剂量的增加,Ca2+-ATP酶和Na+-K+-ATP酶活性被诱导,Ca2+-Mg2+-ATP酶活性被抑制,当Ca2+-Mg2+-ATP酶的抑制程度超过Ca2+-ATP酶和Na+-K+-ATP酶的代偿机制时,会抑制精子的发生,进而导致雄性生殖毒性;在1.6mg·L-1Pb2+处理下,ACP酶被显著抑制,提示支持细胞受损,精子总数受到影响;在0.4mg·L-1Pb2+处理下,LDH酶被显著抑制,提示生精细胞受损,精子质量受到影响.

Effects of low-dose and long-term lead (Pb) exposure on testicular enzymes in male Rana nigromaculata

To evaluate the reproductive toxicity of low-dose and long-term lead(Pb) exposure in male Rana nigromaculata,healthy adult male frogs were exposed to 0.1,0.2,0.4,0.8 and 1.6 mg · L-1 of lead solution for 30 d under experimental conditions.The activities of Ca2+-ATPase,Na+-K+-ATPase,Ca2+-Mg2+-ATPase,β-DG,LDH and ACP were tested.The results showed that the activities of Ca2+-ATPase and Na+-K+-ATPase were induced with the increasing lead level,while Ca2+-Mg2+-ATPase being inhibited.When the decrease of Ca2+-Mg2+-ATPase exceeded the increase of Ca2+-ATPase and Na+-K+-ATPase,the quantity of sperm decreased and the male reproductive toxicity of the frog was induced by lead.In addition,the activities of ACP were inhibited under 1.6 mg · L-1 Pb2+,implying decrease of the quantity of sperm because of damage of sertoli cell.Also,the activities of LDH were significantly inhibited under 0.4 mg · L-1 Pb2+,implying decrease of the quality of sperm because of the damage of spermatogenic cells.