| 有机硒多糖对铬致小鼠细胞DNA损伤与氧化损伤的保护作用 |
| |
| 引用本文： | 铁梅,惠秀娟,张剑慧,陈忠林,王杰,刘晓波,臧树良.有机硒多糖对铬致小鼠细胞DNA损伤与氧化损伤的保护作用[J].环境科学学报,2010,30(1):192-197. |
| |
| 作者姓名： | 铁梅 惠秀娟 张剑慧 陈忠林 王杰 刘晓波 臧树良 |
| |
| 作者单位： | 辽宁大学环境学院; |
| |
| 基金项目： | 国家自然科学基金项目(No.20611130317/B01);;辽宁省自然科学基金项目(No.511050)~~ |
| |
| 摘 要： | 采用单细胞凝胶电泳技术研究了富硒金针菇中提取的硒多糖对铬致小鼠骨髓细胞DNA损伤的保护作用,进而通过考察小鼠内源性脂质过氧化的抑制作用进一步探究硒多糖的生物活性.结果表明:硒多糖在硒含量为0.0244～0.0650μg.mL-1范围内,随浓度和在小鼠体内作用时间的增加,骨髓细胞尾长和尾矩与对照组相比都有显著下降;对小鼠内源性脂质过氧化的抑制作用随浓度的升高而增强,与对照组相比有显著差异.硒多糖作为有机硒对铬致细胞DNA损伤和脂质过氧化均有保护作用,可为新型有机硒补剂的开发和有机硒在食品、药品以及预防科学领域的应用提供科学的理论依据.
|
| 关 键 词： | 硒多糖 脂质过氧化 DNA损伤 单细胞凝胶电泳 |
| 收稿时间： | 4/23/2009 2:59:18 AM |
| 修稿时间： | 6/25/2009 1:45:07 PM |
Protective effect of organic Se-containing polysaccharides against chromium-induced oxidative damage to cell DNA of mice |
| |
| TIE Mei,HUI Xiujuan,ZHANG Jianhui,CHEN Zhonglin,WANG Jie,LIU Xiaobo and ZANG Shuliang.Protective effect of organic Se-containing polysaccharides against chromium-induced oxidative damage to cell DNA of mice[J].Acta Scientiae Circumstantiae,2010,30(1):192-197. |
| |
| Authors: | TIE Mei HUI Xiujuan ZHANG Jianhui CHEN Zhonglin WANG Jie LIU Xiaobo and ZANG Shuliang |
| |
| Institution: | TIE Mei,HUI Xiujuan,ZHANG Jianhui,CHEN Zhonglin,WANG Jie,LIU Xiaobo,ZANG ShuliangSchool of Environmental Science,Liaoning University,Shenyang 110036 |
| |
| Abstract: | The protective effect of Se-polysaccharides extracted from Se-enriched Flammulina velutipes on the bone marrow cell DNA of mice exposed to chromium was studied by single cell gel electrophoresis. In order to evaluate the bioactivity of Se-polysaccharides,their inhibition of endogenous lipid peroxidation of mice was examined. The results showed that both tail length and tail moment of marrow cells,as compared to the control group,decreased markedly with the increase of concentration and action time in mice,w... |
| |
| Keywords: | Se-polysaccharide lipid peroxidation DNA damage single cell gel electrophoresis |
| 本文献已被 CNKI 等数据库收录！ |
| 点击此处可从《环境科学学报》浏览原始摘要信息 |
| 点击此处可从《环境科学学报》下载免费的PDF全文 |
|
