采煤塌陷地抗生素抗性基因污染现状与机理分析

针对采煤塌陷区特点,介绍了抗生素抗性基因(ARG)的环境风险,讨论了ARG污染地下水的机理,分析了当前存在的关键问题和主要研究方向。分析认为:细胞间的水平基因转移促进ARG向地下水迁移,并受抗生素、重金属等多种因素影响;微生物群落结构和环境条件改变也影响着ARG的分布;土壤等多孔介质可滞留部分胞内ARG,而胞外ARG的垂向迁移可导致地下水污染;统一环境介质中ARG的丰度表达方式,可根据所采用的测序技术,将绝对丰度和相对丰度分别表示为copy/cell、PPM;需区分细胞内ARG和细胞外ARG,明确ARG在胞间的动态分配机理,分别开展二者在环境介质的界面吸附与迁移行为研究。

Analysis of status and mechanism of antibiotic resistance gene pollution in coal mining subsidence

According to the characteristics of coal mining subsidence area,the environmental risk of antibiotic resistance gene(ARG)was introduced.The mechanism of groundwater pollution by ARG was discussed.The key problems and main research directions were analyzed.The results show that horizontal gene transfer among cells promotes the transport of ARG toward groundwater.This process is affected by various factors such as antibiotics and heavy metals.The distribution of ARG is affected by microbial community structure and environmental conditions.Intracellular ARG can be partly retained by porous media such as soil,while extracellular ARG will cause groundwater pollution by vertical transport.According to the sequencing technology,the absolute abundance and relative abundance can be expressed as copy/cell,PPM,respectively,to unify the expression of ARG abundance in the environment.It is necessary to distinguish intracellular ARG and extracellular ARG,and clarify the dynamic allocation mechanism among cells.Their adsorption and migration behaviors at the interface of environmental media shoule be further studied.