高效异养硝化细菌Alcaligenes faecalis Ni3-1的分离及其脱氨特性研究

从生活排污渠中分离筛选出高效异养硝化菌株Ni3-1,通过形态和16S r DNA序列分析,初步鉴定为Alcaligenes faecalis。脱氨特性研究表明:Ni3-1的异养硝化作用主要发生在指数期;碳源对菌株脱氨效果影响较大,柠檬酸三钠和丁二酸钠为最佳碳源;在氨氮为10~1 000 mg/L时,Ni3-1均表现出较高的脱氨能力;Ni3-1适应能力较强,温度为25~35℃,p H为6~9,C/N为10~15时,24 h氨氮去除率均达95%以上。将Ni3-1用于处理高氨氮猪场废水,48 h氨氮去除率可达93.2%,且未检测到亚硝态氮和硝态氮的积累。总体而言,菌株Ni3-1在脱氨效率和适应能力方面具有明显优势,在污水脱氮处理中具有一定的开发利用价值。

ISOLATION AND NH4+-N REMOVAL CHARACTERISTICS OF A HIGH-EFFICIENT HETEROTROPHIC NITRIFYING BACTERIUM ALCALIGENES FAECALIS Ni3-1

A high-efficient heterotrophic nitrifying bacterial strain named Ni3-1 was isolated from sewage ditches． Based on morphological observation and 16S rDNA sequence analysis，strain Ni3-1 was identified as Alcaligenes faecalis． NH + 4 － N removal characteristics of strain Ni3-1 was then analyzed． Ｒesults showed that heterotrophic nitrification by the strain Ni3-1 occurred mainly during its logarithmic growth phase． Carbon source had an obviously effect on NH+ 4 －N removal efficiency， and trisodium citrate and sodium succinate were the most appropriate ones． The strain exhibited appreciable heterotrophic nitrification activity when the NH+ 4 －N concentration was in the range of 10 ～ 1 000 mg /L． Strain Ni3-1 was active over a wide range of temperatures ( 25 ～ 35 ℃) ，pH values ( 6 ～ 9) and C/N ratios ( 10 ～ 25) ，and more than 95% of NH + 4 －N could be removed within 24 h under these conditions． Above all，this strain worked well when applied for pig wastewater treatment，and the NH + 4 －N removal ratio reached 93. 2% within 48 h． Neither of NO － 2 －N nor NO － 3 －N was detected in the treatment process． Heterotrophic nitrifier Alcaligenes faecalis Ni3-1 had high efficiency in NH+ 4 － N removal and had potential for treating wastewater with a high NH+ 4 －N load．