厌氧/好氧交替快速筛选聚磷菌及其生理特性的研究

通过厌氧/好氧交替的平板筛选方法,快速的筛选出除磷率高于60%的高效聚磷菌15株,并对其进行16S rDNA和生理生化测定,除2株为芽孢杆菌外,其余均为γ变形菌纲,主要以Klebsiella sp.和Pseudomonas sp.为主.菌种除磷实验表明,聚磷菌除磷能力越高,发酵终点pH值越高.聚磷菌反投加实验表明,与活性污泥52%的除磷率相比,投加后除磷率可达到73.3%,可以有效提高活性污泥的除磷能力.

Quick screening of polyphosphate-accumulating strains and its characteristics

A quick process was proposed to screen PAOs based on the aerobic/anaerobic cultivation. 15 strains, which had over 60% phosphate removal rate, were screened out and then tested by 16S rDNA and biochemical analyses. Except 2 strains of Bacillus sp., the other strains were γ-Proteobacteria, mainly Klebsiella sp. and Pseudomonas sp.. The phosphate removal experiment indicated that the pH values at the end of fermentation increased with the increase in the removal capacity of the PAOs. The phosphate removal rate increased from 52% to 73.3% after adding the PAOs strain screened into active sludge. The addition of the PAOs strain was an effective way to enhance the phosphate removal capacity of activated sludge.