膜电解氢自养MBBR反应器深度转化水中高氯酸盐

建立膜电解电化学氢自养MBBR反应器（移动床生物膜反应器）用于去除水中高氯酸盐，微生物利用阴极电解产生的氢气将高氯酸根还原为氯离子，而后氯离子在阳极发生氧化析氯反应生成活性氯进一步提升出水水质，从而实现高氯酸根的深度转化.利用该反应器研究了高氯酸根的转化过程及相关影响因素，结果表明：进水ClO₄^-浓度为（4.98±0.091）mg/L，维持HRT（水力停留时间）为4h，施加电流由6mA增加至15mA，反应器对高氯酸根的去除率由（39.75±2.09）%增加至（98.99±0.05）%，总出水活性氯浓度由（0.057±0.003）mg/L增加至（0.070±0.002）mg/L，pH值稳定在7.96~8.11，浊度较低为（0.89±0.27）NTU.进一步增大施加电流（20mA），导致阴极室溶液pH值超过9.5，进而影响微生物活性，去除率急剧下降至（30.75±1.19）%.利用扫描电子显微镜（SEM）观察反应器内微生物形貌，发现反应器内微生物均附着于填料表面，以短杆菌为主，增殖缓慢.运用高通量测序技术对接种及运行第24d的微生物群落结构展开分析.结果显示，反应器运行过程中，菌群多样性下降，Thauera菌属为主要的氢自养还原优势菌属，其丰度达到8.25%.

Conversion of perchlorate from aqueous solution by hydrogen autotrophic MBBR reactor based on proton-exchange membrane electro-dialysis system

Based on membrane electro-dialysis system, a novel hydrogen autotrophic MBBR (Moving Bed Biological Reactor) was established to remove perchlorate from aqueous solution. In cathode chamber, bacteria could utilize hydrogen as electron donor to transform perchlorate to chloride ion; in anode chamber, the chloride ion was oxidized to active chlorine to improve effluent quality. The conversion of perchlorate and the influential factors were explored. The hydraulic retention time (HRT) was maintained at 4h. For 4.98±0.091mg/L ClO₄^- in feed water, when current intensity increased from 6to 15mA, the removal efficiency increased from (39.75±2.09)% to (98.99±0.05)% correspondingly. The active chlorine increased from 0.057±0.003 to 0.070±0.0002mg/L. The pH of effluent was kept at 7.96~8.11 and the turbidity was 0.89±0.27NTU. When further increase current intensity to 20mA, the pH of cathode chamber exceeded 9.5 thus inhibiting the activity of microorganism and the removal efficiency decreased sharply to (30.75±1.19)%. Besides, scanning electron microscope (SEM) was used to analyze the morphology of bacteria. The dominant bacterial was mainly short rod-shaped, attached to the surface of carriers and proliferated slowly. High-throughput sequencing was applied to analyze bacterial samples after inoculation and 24d operation. The results indicated that the α-biodiversity was decreased during the operation time and the Thauera was the dominated bacteria with 8.25% abundance.