温度对FBAR反应器的运行特性及古菌微生物群落影响

为探讨固定床厌氧反应器（FBAR）在不同温度下的运行特性及微生物群落变化，比较了高温（50℃）、中温（35℃）、低温（4℃）3个温度阶段反应器产甲烷特性及古菌群落变化.结果表明；绝对产气量由大至小依次为高（50℃）、中（35℃）、低温（4℃），单位负荷产气量依次为中温（2.84L/OLR），低温（2.5L/OLR），高温（1.8L/OLR）；甲烷含量依次为低温（74.5%）、中温（63.5%），高温（57.3%），不同温度阶段对挥发性有机酸含量变化有一定的影响.克隆文库分析表明：不同温度条件下固定床厌氧反应器内部微生物群落的丰富性存在很大的差异.定量PCR分析表明：甲烷鬃毛菌是中温和高温反应器内的优势菌，低温4℃炭纤维载体和污泥中的优势菌都是甲烷微菌.从能耗、经济效益角度分析低温条件更适合沼气发酵，而主要是以嗜氢产甲烷菌代谢途径为主.

Comparison to the characteristics and archaea communites under different temperature stages of thermophilic,mesophilic and psychrophilic of fixed bed anaerobic reactor

In order to investigate the change of characteristics and methanogenic microorganisms under three temperature stages. Comparison to the dynamic of methane character and archaea communites under three temperature stages of thermophilic (50℃), mesophilic(35℃) and psychrophilic(4℃). The result showed that the order of absolute biogas production from high to low was thermophilic (50℃), mesophilic(35℃) and psychrophilic(4℃). The order of biogas production mesophilic, psychrophilic and thermophilic was 2.84, 2.5 and 1.8L/OLR, respectively. The order of methane content psychrophilic, mesophilic and thermophilic was 74.5, 63.5 and 57.3%, respectively. The volatile organic acids content was a big influence under different temperature stage. Clone library analysis that the diversity of microbiology was significant difference within different temperature stage. Quantitative PCR analysis that the methanosaetaceae was the dominant methanogens within 50℃ and 35℃reactors, the methanomicrobiales was the dominant methanogens in the adhering sludge at 4℃. From energy consumption an economic benefit analysis that the biogas fermentation was more suitable under low temperature, and the main metabolic pathways was hydrogenotrophic methanogenesis.