餐厨垃圾干式厌氧消化污泥膨胀微生态特征

分别在推流式反应器（PFR）（R1）和完全混合式反应器（CSTR）（R2）中进行餐厨垃圾（KW）中温干式厌氧消化（AD），并采用MiSeq高通量测序技术分析反应器污泥膨胀前后的微生态特征.结果表明，污泥膨胀后古菌群落结构变化不显著，R1和R2分别以乙酸营养型的Methanosaeta和复合营养型的Methanosarcina为优势产甲烷菌；而细菌群落中某些可能与污泥膨胀有关的菌属的相对丰度显著增大，包括能合成并分泌生物表面活性物质的菌属（如Corynebacterium、Lactobacillus等）和细胞壁含分枝菌酸的菌属（如Actinomyces、Corynebacterium）.反应器在污泥膨胀前均经历了挥发性脂肪酸（VFAs）和氨氮积累，相应的，能贡献系统酸积累（如Petrimonas、Anaerosalibacter和Fastidiosipila等）和氨氮积累（如Proteiniphilum、Tepidimicrobium和Aminobacterium等）的菌属也大量增殖.

Microbial characteristics of bulking sludge in high-solids anaerobic digestion of kitchen waste

A plug-flow reactor (PFR)(R1) and a completely-stirred tank reactor (CSTR)(R2) were operated under mesophilic temperature (37±1℃) for high-solids digestion of kitchen waste to investigate the microbial community characteristics before and after sludge-bulking using MiSeq high-throughput sequencing technology.The results showed that the archaeal community structure changed little after sludge-bulking,and acetoclastic methanogen Methanosaeta and mixotrophic methanogen Methanosarcina dominated in both reactors.There was a marked increase in the relative abundance of bacteria genera that might be related to sludge bulking.Those proliferated bacteria genera are those capable of producing biosurfactants (Corynebacterium,Lactobacillus,etc.) and those containing mycolic acids in cell walls (Actinomyces,Corynebacterium,etc.).In addition,volatile fatty acids (VFAs) and total ammonia nitrogen (TAN) were accumulated in these reactors before sludge-bulking.Accordingly,bacteria that can contribute to the accumulation of VFAs (Petrimonas,Anaerosalibacter and Fastidiosipila,etc.) and TAN (Proteiniphilum,Tepidimicrobium and Aminobacterium,etc.) were observed to proliferate.