一株苯胺降解菌的筛选及处理突发污染的效果

从污水厂活性污泥中分离到1株能快速、高效降解苯胺的菌株AN-P1,鉴定为Rhodococcus sp..相关酶活性的测定表明,苯胺通过间位途径降解.菌株AN-P1利用苯胺生长的最适温度30℃,最适pH值为6.0,最适生长浓度为2000 mg/L,最佳接种量为0.30‰.将AN-P1作为功能菌株对常规活性污泥系统强化后应急处理,16S rRNA基因V3区片段PCR-DGGE图谱显示,AN-P1能有效保护原有生态系统中的微生物免受苯胺毒害;处理含500,1000,2000mg/L苯胺的模拟废水分别需10,20,32h就能使出水水质达到《污水综合排放标准》一级标准;用AN-P1结合常规SBR工艺应急处理苯胺废液,经过36h降解能达标排放.

Isolation of an efficient aniline-degrading bacterial strain and its application for accidents

A bacterial strain AN-P1 capable of efficient aniline-degrading was isolated from activated sludge of a sewage treatment plant. It was identified as Rhodococcus sp.. Enzymatic analysis showed that aniline was degraded through meta-cleavage pathway. The optimal conditions for cell growth and aniline degradation were pH 6, 30℃ with an inoculation amount of 0.30‰ and an aniline concentration of 2000 mg/L. PCR-DGGE profile showed that AN-P1 could efficiently protect the microbial community of the activated sludge system against sudden aniline pollution. It took only 10,20, 32h, respectively,for the treatment of wastewater containing 500, 1000, 2000mg/L aniline to meet the first grade of national integrated wastewater discharge standard. With a combination of AN-P1 and SBR system,it took only 36h for the emergency treatment of wastewater containing 2000 mg/L aniline to meet the discharge standard.