双酚F和双酚S联合暴露下的斑马鱼富集及神经毒性

为探究双酚F（BPF）和双酚S（BPS）联合暴露下斑马鱼富集及神经毒性，进行为期14d的1，10，100，1000 μg/L BPF和BPS的单一或联合暴露.结果表明，BPF和BPS在斑马鱼组织内的富集水平与暴露浓度和暴露时间呈正相关，BPF富集能力高于BPS，BPF和BPS联合暴露降低了斑马鱼对BPS的富集水平，但对BPF富集水平的影响较小.脑组织中的富集水平低于肠道但远高于肌肉.以脑为目标组织进行了毒性研究，结果表明，BPF诱导的丙二醛（MDA）、8-羟化脱氧鸟苷（8-OHdG）、白细胞介素1β（1L-1β）、肿瘤坏死因子α（TNF-α）等氧化损伤和炎症指标水平高于BPS，且联合暴露存在毒性增强效应.但BPS对皮质醇（COR）、肾上腺素（EPI）和乙酰胆碱酶（AChE）等神经毒性标志物和Syn2a、MBP、Gfap、MAP2、NSE2和S-100B等神经功能有关基因的毒性效应影响高于BPF，联合暴露削减了对AChE、COR和EPI等的影响.

ccumulation and neurotoxicity of bisphenol F and bisphenol S in zebrafish under combined exposure

In orderto investigate the enrichment of bisphenol F (BPF) and bisphenol S (BPS) in zebrafish and their combined neurotoxicity, single and combined exposure of 1, 10, 100, 1000μg/L BPF and BPS was conducted for 14days. The enrichment level of BPF and BPS in zebrafish tissues was positively correlated with the exposure concentration and time, and the enrichment capacity of BPF was higher than that of BPS. Combined exposure of BPF and BPS reduced the enrichment level of BPS in zebrafish, but had little effects on the enrichment of BPF. Toxicity studies on brain tissues showed that BPF induced levels of oxidative damage and inflammation indicators such as malondialdehyde (MDA), 8-hydroxy-2'-deoxyguanosine (8-OHdG), interleukin-1β(1L-1β) and tumor necrosis factor α (TNF-α), were higher than those of BPS. Moreover, combined exposure was found to have toxicity enhancement effects. However, the toxic effects of BPS on neurotoxic markers, such as cortisol (COR), epinephrine (EPI) and acetylcholinesterase (AChE), and genes related to neurological functions, such as Syn2a, MBP, Gfap, MAP2, NSE2 and S-100B, were higher than those of BPF. And, combined exposure reduced the effects on AChE, COR and EPI.