异养硝化-好氧反硝化菌HY3-2的分离及脱氮特性

从经过高盐驯化的好氧颗粒污泥系统中筛选出一株异养硝化-好氧反硝化菌HY3-2，通过形态学观察及16S rDNA序列分析得出HY3-2为Klebsiella quasipneumoniae subsp.quasipneumoniae.研究了HY3-2对氨氮、硝酸盐和亚硝酸盐的去除特性，结果表明该菌具有良好的异养硝化和好氧反硝化功能，对氨氮、硝酸盐和亚硝酸盐的去除率分别达63.57%、88.11%和98.38%.对菌株脱氮性能研究表明：HY3-2以甘油为碳源，C/N为25，温度为20℃或30℃，转速为150r/min，盐度低于50g/L时，对100mg/L的NH₄⁺-N去除效果良好，去除率达90.7%；以柠檬酸钠为碳源，C/N为25，温度为30℃，转速为150r/min，盐度低于15g/L时能进行良好的好氧反硝化作用，NO₃^--N去除率达99%以上.

Isolation and denitrification characteristics of heterotrophic nitrification-aerobic denitrification bacterium HY3-2

A heterotrophic nitrification-aerobic denitrification bacterium named HY3-2 was isolated from the high-salt aerobic granular sludge system. The morphological observation and 16S rDNA sequence analysis showed that HY3-2 was Klebsiella quasipneumoniae subsp.quasipneumoniae. The removal characteristics of ammonia nitrogen, nitrate and nitrite by HY3-2 were studied. The results showed that the strain had good heterotrophic nitrification and aerobic denitrification performance, and the removal rates of ammonia nitrogen, nitrate and nitrite were 63.57%, 88.11% and 98.38% respectively. The study on the nitrogen removal performance of the strain showed that:HY3-2 used glycerol as carbon source. Under the condition (C/N 25, temperature 20℃ or 30℃, rotation speed 150r/min, initial pH 7~10, salinity less than 50g/L), the effect on NH₄⁺-N removal with the concentration of 100mg/L was good, and the removal rate was 90.7%. When the sodium citrate was used as carbon source (C/N 25, temperature 30℃, rotation speed 150r/min, initial pH 5~9), the aerobic denitrification can be performed when the salinity was less than 15g/L, the NO₃^--N removal rate was over 99%.