亚油酸对铜绿微囊藻的抑制机理

为有效利用化感物质控制水华藻类,实验探讨了亚油酸对铜绿微囊藻的抑制机理.结果表明,采用亚油酸处理铜绿微囊藻后,铜绿微囊藻的叶绿素a含量下降、细胞外液中电导率、OD260增加;细胞O2 、MDA产生增多; SOD酶活力和比活力、谷胱甘肽-过氧化物酶(GSH-Px)酶活力在前4d内应激性升高,SOD同工酶的谱带在亚油酸浓度为0.1mg/L时表达增强.在亚油酸抑藻的实验组中同时加入抗氧化剂维生素C后, O2 的产生减少,藻生物量增加.因此推测亚油酸的抑藻机理可能是通过O2 的产生,引起细胞质膜的膜质过氧化,造成细胞膜通透性的改变,并导致DNA等生物大分子的损伤,从而抑制了叶绿素a以及有关蛋白质的合成,最终使藻细胞死亡与溶解.

The inhibitory mechanism of linoleic acid on Microcystis aeruginosa

In order to effectively control water-bloom algae by using allelochemicals, the inhibitory mechanism of linoleic acid on M. aeruginosa was analyzed. After the linoleic acid added in the experimental groups, the chlorophyll a content of algal cells decreased, the electrical conductivity (EC) and the OD260 of algal extracellular fluid increased, the generation of malondialdehyde (MDA) and superoxide anion radical (O2 ) of the algal cells increased, activities of superoxide dismutase (SOD), glutathione peroxidase (GSH-Px) increased at the first 4 days and the expression of SOD isoenzyme enhanced at the concentration of linoleic acid being 0.1mg/L. But when antioxidants vitamin C was added the generation of O2 decreased, and algal biomass increased. All the results indicated that the mechanisms underlying the algal growth inhibition by linoleic acid involved the O2 generation that induced the lipid peroxidation of cell membrane, changed the penetrability of cell membrane, and resulted in the damage of DNA and some bio-macromolecules, inhibited the synthesis of the chlorophyll a and some relative proteins, breaking the algal cells and causing them melt in the end.