壬基酚对一株铜绿假单胞菌吸附镉的影响

考察壬基酚（NP）对一株从重金属污染场地筛选出的铜绿假单胞菌X吸附镉（Cd）的影响，在NP浓度分别为0、1.0、10.0mg/L条件下，通过优化吸附条件，研究X菌的Cd²⁺吸附效果.结果表明，NP与Cd²⁺共存时，在Cd²⁺初始浓度为1.0mg/L的溶液中，菌体的最佳投菌量和pH值为1.0g/L和7.0，吸附2h，吸附率可达90.0%左右.失活菌与活菌的吸附结果表明失活菌的吸附能力较活菌强.NP浓度对吸附的影响结果表明，低浓度NP对菌株吸附Cd²⁺的抑制作用较小，高浓度NP时抑制作用较大.通过分析X菌处理单一Cd²⁺及Cd²⁺-NP复合污染后的红外光谱图可知，菌体表面的羟基O-H键、酰胺C-N键和N-H键和氨基均参与吸附反应，并且高浓度NP对菌体表面基团活性影响较大，从而影响其对Cd²⁺的吸附.

Biosorption of cadmium in nonylphenol-cadmium compound pollution by Pseudomonas aeruginosa X

Effects of nonylphenol (NP) on the adsorption of cadmium by Pseudomonas aeruginosa X, which is screened from cadmium (Cd) contaminated site was investigated. Experiments were undertaken to study the biosorption of cadmium at NP concentrations of 0、1.0、10.0mg/L respectively. It was revealed that the optimal concentration of Pseudomonas aeruginosa X and pH were 1.0g/L and 7.0respectively with initial Cd²⁺ concentration of 1.0mg/L and hybrid with NP. With two hours absorbing, the biosorption rate was about 90 %. The results of biosorption by devitalized bacteria and live bacteria indicated that the adsorption ability of devitalized bacteria was better than that of live bacteria. By considering the different concentrations of NP as variables, it was found that the lower concentration of NP has little suppressing effect on the adsorption of Cd²⁺. But the suppressing effect was significant at higher concentrations of NP. FTIR spectra of bacteria X treating Cd²⁺ and compound of Cd²⁺-NP separately indicated that the functional groups of hydroxyl group and amido were involved in the adsorption reaction. Moreover, it indicates that high concentration of NP has a greater impact on the activity of cell surface groups. Thus, high concentration of NP affects the adsorption of Cd²⁺.