硝化污泥中AOB/NOB对硝化特性的影响

为了深入研究硝化污泥中AOB/NOB（A/N）对其硝化特性的影响，在两个SBR反应器中，通过控制单一基质，并结合影响因素控制和定期排泥，各自经过80个周期的运行，成功实现了AOB、NOB活性污泥的优化培养.依据Monod方程理论确定出AOB、NOB活性污泥中的丰度比约为1：1.不同A/N硝化特性的研究表明：亚硝化率、氨氧化速率、亚硝酸盐氧化速率以及好氧速率均受硝化污泥中A/N的影响，想要实现短程硝化的稳定运行必须使得A/N接近于1：0；氨氧化速率与硝化污泥中AOB的数量并不存在显著的正比关系；常规的生活污水硝化工艺中，A/N应不低于1：2；结合好氧速率的在线监测，当好氧速率趋于稳定时，指示短程硝化的启动已经接近完成.

The effect of AOB/NOB in nitrifying sludge on nitrification characteristics

The effect of AOB/NOB in nitrifying sludge on nitrification characteristics was deeply investigated in this study. Firstly, activated sludge only with AOB and NOB was successfully cultivated in two same SBRs through 80 cycles, respectively. The main substrate in their inflow was single, during cultivation influencing factors were controlled and sludge was discharged at regular intervals. Secondly, the ratio of abundance between AOB sludge and NOB sludge was determined through experiment which was about 1:1. Finally, experiment results about nitrification characteristics showed that nitrosation rate, ammonia oxidation rate, nitrite oxidation rate and oxygen uptake rate were influenced by A/N in nitrifying sludge. A/N should be close to 1:0 for steady operation of partial nitrification; there was no obvious positive relationship between ammonia oxidation rate and proportion of AOB in nitrifying bacteria; A/N in conventional nitrification sludge for municipal sewage treatment should not less than 1:2; combined with online monitoring for oxygen uptake rate, when it became stable, the startup of partial nitrification could be considered as nearly achieved.