PCR-DGGE技术分析塔式蚯蚓生态滤池微生物群落结构

利用PCR-DGGE技术研究塔式蚯蚓生态滤池微生物群落结构变化,结果表明,微生物的变化与滤池体积大小、水力停留时间和污染物负荷有关,微生物群落结构在不同滤池层中变化较大.12个样品泳道中的总细菌的Shannon-Wiener多样性指数从0.25变化到1.27,一级滤池微生物多样性指数从0.29变化到1.05,二级滤池微生物多样性指数从0.75变化到0.97,三级滤池微生物多样性指数先从0.87减少到0.25,后由于葡萄糖的加入增加到1.27.二级滤池出水中葡萄糖的加入,加强了系统内微生物的活性,从而三级滤池砂石和青石层细菌条带变亮变多.经过克隆测序和系统发育树分析,塔式蚯蚓生态滤池内的微生物多为单细胞菌属、假单细胞菌属、革兰氏阴性菌属和不可培养杆菌属,不同细菌条带在不同级滤池内差异变化明显.

Research on microbe community in tower earthworm ecology-filter by PCR-DGGE

Change of microbe community in tower earthworm ecology-filter was studied in this research with PCR-DGGE technology (polymerase chain reaction-denaturing gradient gel electrophoresis). These results showed that: the change of microbe community was obvious and linked with hydraulic resident time、pollutation burden and the volume of filters. Meanwhile, Shannon-Wiener Index of biodiversity increased from 0.25 to 1.27 in an all-round way, including 0.29～1.05 in the first filter, 0.75~0.97 in the second filter and 0.87~0.25~1.27 caused by some glucose added into influent in the last filter. Under the same reason, the activity and light of denitrifying bacteria were intensified on bluestone and gravel layers. After analysis of cloning sequencing and phylogenetic tree, most of the inferred bacteria were Sphingomonas, Pseudomonas, Gram-negative bacteria and uncultured Bacillus and changed evidently in different filters.