氯氰菊酯对小鼠脑细胞的氧化损伤及维生素E的抗氧化作用

以昆明小鼠为受试动物,随机分为6组,包括1个阴性对照组、3个氯氰菊酯染毒组、1个维生素E组和1个高剂量氯氰菊酯加维生素E组,染毒组按10,20,40mg/kg 3个剂量水平,维生素E的剂量为100mg/kg,灌胃染毒小鼠7d.以脑组织匀浆测定活性氧(ROS)、还原型谷胱甘肽(GSH)和丙二醛(MDA)的含量;以脑组织细胞测定DNA-蛋白质交联(DPC)系数.随着氯氰菊酯染毒剂量的升高,脑组织的ROS、MDA含量和DPC系数逐渐上升,GSH含量逐渐降低,各指标呈一定的剂量-效应关系.染毒剂量320mg/kg时,ROS含量(841.3±100.34)、GSH含量(12.54±1.316)nmol/L]和DPC系数(0.054±0.004)有显著差异(P<0.05);染毒剂量340mg/kg时,GSH含量(10.51±1.545)nmol/L]有显著差异(P<0.05),ROS含量(1014.3±81.67)、MDA含量(2.849±0.218)μmol/L]和DPC系数(0.079±0.005)有极显著差异(P<0.01).高剂量染毒加维生素E组与高剂量染毒组相比较,脑组织的ROS、MDA含量和DPC系数均有下降,GSH含量上升.脑组织的ROS(719.5±74.56)、GSH(16.52±1.985)nmol/L]和DPC系数(0.055±0.005)有显著差异(P<0.05),MDA含量(1.662±0.265)μmol/L]有极显著差异(P<0.01).较高剂量(320mg/kg)的氯氰菊酯能造成小鼠脑组织的氧化损伤,维生素E有抗氧化作用.

Oxidative damage of pesticide cypermethrin on mouse brain cells and the antioxidant role of vitamin E

Kunming mice were randomly grouped into six groups and orally administered with drugs daily for a week; the groups included one solvent control group, three cypermethrin groups, one high dose cypermethrin plus vitamin E protection group and one vitamin E group. The exposure doses of cypermethrin groups were 10, 20and 40mg/kg respectively. Some brain tissues were then made into homogenates for the measurement of ROS (reactive oxygen species), GSH (glutathione) and MDA (Malondialdehyde) contents. Meanwhile, DPC (DNA-protein Crosslink) coefficients were detected from brain cell suspension. The brain contents of ROS, MDA and DPC coefficients increased gradually in a dose-dependent manner, whereas GSH content decreased accordingly. In the exposure group with the dose of 20mg/kg, ROS(841.3±100.34), GSH contents(12.54±1.316)nmol/L] and DPC coefficients(0.054±0.004) were significantly higher than those of the control group (P<0.05); In the highest dose group (40mg/kg), GSH content (10.51±1.545)nmol/L]was significantly higher than that of the control group (P<0.05), the significance of differences of ROS(1014.3±81.67)、MDA contents(2.849±0.218)μmol/L] and DPC coefficients(0.079±0.005) to the control group have all become extreme (P<0.01). However, compared to the high cypermethrin dose group, high dose cypermethrin plus vitamin E protection group had lower ROS(719.5±74.56 )(P<0.05), MDA(1.662±0.265)μmol/L] (P<0.01) and DPC coefficients(0.055±0.005) (P<0.05) levels, and higher GSH contents(16.52±1.985)nmol/L](P<0.05). Cyermethrin at certain doses (320mg/kg) can induce oxidative stress in mice brain, whereas vitamin E has antioxidant effects.