| 醋酸钙不动杆菌PHEA-2对苯酚的降解特性研究 |
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| 引用本文： | 陈明,张维,徐玉泉,平淑珍,陆伟,林敏.醋酸钙不动杆菌PHEA-2对苯酚的降解特性研究[J].中国环境科学,2001,21(3):0-0. |
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| 作者姓名： | 陈明 张维 徐玉泉 平淑珍 陆伟 林敏 |
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| 作者单位： | 1.中国农业科学院原子能研究所 北京100094;2.中国农业大学生物学院 北京100094 |
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| 基金项目： | 863计划资助课题(863-SZ-03-01) |
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| 摘 要： | 自炼油厂污水中分离的醋酸钙不动杆菌(Acinetobacter calcoaceticus) PHEA-2具有 较强的苯酚降解能力.在温度为30℃和接种量为1%条件下,该菌在24h内完全降解苯酚的浓度 为300mg/L；该菌的最适生长和最适降解苯酚温度为30℃;在pH值5～10范围内能保持对苯酚的 降解能力.醋酸钙不动杆菌PHEA-2的苯酚降解反应符合Monod动力学模式,其反应的米氏常数K m为275mg/L,最大反应速度Vm为41mg苯酚/(108·h).一定量的葡萄糖(5%)促进PHEA-2菌体的 生长,从而提高了整个培养体系降解苯酚的能力.
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| 关 键 词： | 醋酸钙不动杆菌 生物降解 苯酚 |
| 文章编号： | 1000-6923(2001)03-0226-04 |
| 收稿时间： | 1900-01-01; |
| 修稿时间： | 2000年9月26日 |
Study on characteristics
of Acinetobater calcoaceticus PHEA-2 for phenol degradation |
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| CHEN Ming,ZHANG Wei,XU Yu-quan,PING Shu-zhen,LU Wei,LIN Min.Study on characteristics
of Acinetobater calcoaceticus PHEA-2 for phenol degradation[J].China Environmental Science,2001,21(3):0-0. |
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| Authors: | CHEN Ming ZHANG Wei XU Yu-quan PING Shu-zhen LU Wei LIN Min |
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| Institution: | CHEN Ming1,ZHANG Wei1,XU Yu-quan1,2,PING Shu-zhen1,LU Wei1,LIN Min1 |
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| Abstract: | Acinetobacter calcoaceticus PHEA-2 separated from the wastewater of oil refinery has rather strong ability of phenol degradation. Under the conditions of 30℃ and incubation amount of 1%, it can degrade 300mg/L phenol entirely within 24 hours; and the optimal temperature for its growth and phenol degradation is 30℃. The degradation ability can maintain at the range from pH5 to pH10. The degrading reaction of Acinetobater calcoaceticus PHEA-2 accords with Monod model. The reaction constant Km is 275mg/L; and the greatest reaction speed Vm is 41mg phenol/(108·h). Glucose applied at 5% can stimulate the growth of PHEA-2 and enhance the degrading ability of PHEA-2 culture system. |
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