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基于微电极技术的反硝化滤池生物膜特性分析
引用本文:宋慧敏,周小红,张永明,施汉昌.基于微电极技术的反硝化滤池生物膜特性分析[J].中国环境科学,2012,32(5):850-854.
作者姓名:宋慧敏  周小红  张永明  施汉昌
作者单位:1. 清华大学环境学院,北京100084;上海师范大学生命与环境科学学院,上海200235
2. 清华大学环境学院,北京,100084
3. 上海师范大学生命与环境科学学院,上海,200235
基金项目:国家水体污染控制与治理科技重大专项(2011ZX07301-002)
摘    要:为研究反硝化滤池中溶解氧对反硝化作用的影响,制备性能良好尖端直径在30μm以内的氧(O2)以及硝酸盐(NO3-)微电极,以此为测试工具,对反硝化滤池中生物膜内部O2、NO3-微环境分布进行测试,通过建立扩散-反应方程,获得生物膜微环境耗氧及反硝化活性特征.研究结果表明,溶解氧在生物膜内部呈明显的下降趋势,从主体溶液氧浓度约1mg/L下降至生物膜300μm深度处约为0.生物膜内部反硝化活性区域发生在300~600μm深度范围内.该条件下反硝化滤池生物膜的氧利用速率常数以及反硝化速率常数之间的比值为1.46,溶解氧对反硝化过程的影响是显著的.

关 键 词:生物膜  反硝化滤池  微电极    硝酸盐  
收稿时间:2011-09-20;

Characterization of biofilm in denitrification biofilter based on microelectrode technique
SONG Hui-min , ZHOU Xiao-hong , ZHANG Yong-ming , SHI Han-chang.Characterization of biofilm in denitrification biofilter based on microelectrode technique[J].China Environmental Science,2012,32(5):850-854.
Authors:SONG Hui-min  ZHOU Xiao-hong  ZHANG Yong-ming  SHI Han-chang
Institution:1(1.School of Environment,Tsinghua University,Beijing 100084,China;2.School of Life and Environmental Sciences,Shanghai Normal University,Shanghai 200235,China)
Abstract:In order to study the impact of dissolved oxygen(DO) on denitrification process in denitrification biofilter,microelectrodes with tip diameters of 30μm were fabricated to determine the microprofiles of O2 and NO3-in biofilms.Based on the profiles,a diffusion-reaction model was established to evaluate the microbial activities in terms of utilization rates of oxygen and nitrate.The results showed that the concentration of DO decreased rapidly along the depth of biofilm,which decreased from 1 mg/L in the bulk solution to almost 0 mg/L at 300μm depth of biofilm.Denitrification occurred in the depth zone of 300~600μm in the biofilm,and the ratio of oxygen uptake rate constant to denitrification rate constant was 1.46,which indicated the remarkable impact of DO on the denitrification process in biofilms.
Keywords:biofilm  denitrification biofilter  microelectrode  oxygen  nitrate
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