原生质体紫外诱变提高简青霉的木质素降解性能

以简青霉Penicillium simplicissimum (Oudem.) Thom BGA为出发菌株,对其进行原生质体紫外诱变.经过筛选获得2株诱变菌株(J12与J18),它们的木质素降解酶酶活要明显高于出发菌株J.其中诱变菌株J12产生的漆酶和锰过氧化物酶酶活较高,相对于出发菌株J分别提高了145%和47%,达到44.0,50.9 U/g.诱变菌株J18产生的木质素过氧化物酶酶活较高,达到67.1 U/g,相对于出发菌株J提高了40%.且木质素降解率也最高,相对于出发菌株J分别提高了198.1%.经过7次传代, J12和J18的木质素降解酶活性保持稳定,没有降低.

Improving lignin degradation ability of Penicillium simplicissimum by UV induced protoplast mutagenesis

The aim of this study was to obtain new Penicillium simplicissimum mutants with high yield of ligninolytic enzymes.UV induced mutagenesis of protoplast was performed on original strain Penicillium simplicissimum(Oudem.) Thom BGA.Two genetically stable mutant strains J12 and J18 were selected from a large amount of the regenerative mutants.The highest laccase and manganese peroxidase(Mnp) activities produced by strain J12 were 1.45 and 0.47-fold higher than that by original strain,reached 44.0 U/g and 50.9 U/g,respectively.The highest lignin peroxidase(LiP) activity of 67.1 U/g was obtained by strain J18,which was 0.4-fold increased compared with its original strain.The highest degradation rate of lignin also was obtained by strain J18,which increased 1.98-fold compared with its original strain.Further experiment confirmed after seven generations successively propagating the activity of strain J12 and strain J18 were stable and did not decrease generally.