量子点-Cu2+对L02细胞的联合毒性及NAC的防护作用

以人胚肝细胞(L02)为研究对象,通过研究量子点及Cu2+ (低毒浓度)联合对细胞存活率的影响以及细胞形态的变化确认细胞的毒性,结合抗氧化剂NAC的防护效果探讨复合毒性的氧化损伤机理.结果表明,无论单独Cu2+还是量子点-Cu2+处理组,L02细胞的增殖能力均受到抑制,量子点-Cu2+处理组表现出更加显著的抑制效果,相对与单独Cu2+处理组细胞存活率最大下降300%.经过NAC预处理的细胞在形态和存活率上都显著恢复.说明了安全浓度范围的量子点的与Cu2+共存提高了细胞毒性风险,NAC能够防护量子点与Cu2+单独或联合引起的氧化损伤.

Combined toxicity of QD-Cu2+ on L02 cells and protective effect of NAC.

The oxidative injury of individual QDs or Cu2+ to hepatic cells were both reported,the combined toxicity is concerned due to the co-existence of these two chemicals in human liver.We thus employed human hepatic L02 cells to test the combined toxicity of QDs and Cu2+(lower toxicity) through the cell viability decrease and cell morphology changes,meanwhile,the protective effect of antioxidant reagent NAC was used to evaluate the oxidant injury associated toxicity mechanism.The results showed that both QDs and Cu2+ could decrease the cells viability,and addition of IC10 of QDs could significantly improve Cu2+ induced cell toxicity with cell viability decrease up to 300 %.NAC showed remarkable protective effects with almost identical cell viability and cell morphology compared to the control,indicating the coexistence of lower toxicity of QDs could improve Cu2+ induced L02 cells toxicity,and NAC could effectively decrease QDs-Cu2 induced oxidant injury toxicity.