萘对海洋三角褐指藻生长的毒性效应及生化指标研究

选择在海洋中分布广泛的浮游植物海洋硅藻门的三角褐指藻(Phaeodactylum tricomutum) 作为目标生物,研究了不同萘浓度水平对海洋微藻的生态毒性效应,并同步监测了萘的真实浓度.结果表明,在0~168h内,与对照相比,低浓度萘(初始浓度为0.048~2mg/L)处理组藻的吸光度以及叶黄素、胡萝卜素含量无明显差异.但当萘处理初始浓度达到8mg/L甚至更大时,吸光度、叶黄素和胡萝卜素含量随萘浓度增加而迅速下降,呈现一定的浓度-效应关系.随萘浓度的升高,各处理组中藻类体内超氧化物歧化酶(SOD)活性呈现先升高后下降的“钟形曲线”趋势,而藻类脂质过氧化产物丙二醛(MDA)的含量则呈现先略降低而后急剧上升的趋势.

Toxic effects of naphthalene on the growth of Phaeodactylum tricomutum and relevant biochemical indexes.

Phaeodactylum tricomutum was selected as target organism to study the toxicity of naphthalene under various concentration.From 0h to 168h,the optical absorbance,xanthophyll and carotene content had no obvious difference with blank control in low-concentration groups of naphthalene(0.048~2mg/L).However,when the concentration of naphthalene reached 8mg/L or more,dramatically decreases of the optical absorbance,xanthophyll and carotene content were gained.Activities of superoxide dismutase(SOD) increased at first and then decreased remarkably with the increasing concentration of naphthalene,showed as "Bell Shaped Curve".And the content of malondialdehyde(MDA) decreased slightly and then flared up and lipid peroxidant aggregated with the increasing concentration of naphthalene.