基于提高乙醇产率的常压室温等离子体微藻诱变育种

以普通小球藻FACHB-25为研究对象，利用常压室温等离子体在不同功率条件下对其诱变处理，在功率100W、120W条件下筛选出3株优势藻株，依次编号为S100-7、S120-4、S120-9.其中S120-9藻株培养末期生物量增加明显且多糖产量是原始藻株产量的1.34倍，达到237.98mg/L；S120-4碳水化合物含量占比为37.55%，较原始藻株提高了43.48%.对比了各藻株在光合性能、细胞组分以及细胞形态等方面差异.通过同步糖化发酵（SSF），碳水化合物含量最高的S120-4藻株乙醇产量达到1.58g/10g藻，但其生物量积累受到限制.考虑生物量积累情况，推算出S120-9藻株单位体积藻液乙醇产量最高达到0.1033g/L.

Mutation breeding of microalgae by atmospheric and room temperature plasma based on improving ethanol yield

Chlorella vulgaris FACHB-25 was mutated by atmospheric and room temperature plasma under different power conditions. Three dominant strains were screened out under the power of 100W and 120W, and they were numbered S100-7, S120-4 and S120-9 in turn. Among them, the biomass of the S120-9 algae strain increased significantly at the end of the period, and the polysaccharide production was 1.34times that of the original algae strain, reaching 237.98mg/L; the carbohydrate content of S120-4 accounted for 37.55%, which was 43.48% higher than the original algae strain. The differences of photosynthetic performance, cell composition and cell morphology among different strains were compared. Through simultaneous saccharification and fermentation (SSF), the ethanol yield of S120-4 strain with the highest carbohydrate content reached 1.58g/10g, but its biomass accumulation was limited. Considering the biomass accumulation, the highest ethanol yield per unit volume of S120-9 was 0.1033g/L.