菌株Cupriavidus sp. DT-1对液体和土壤中TCP的降解

采用液质联用（HPLC-MS）的方法检测菌株Cupriavidus sp.DT-1降解2-羟基吡啶（2-HP）的代谢产物.并用三亲结合、荧光定量PCR （q-PCR）方法评价降解菌对3，5，6-三氯-2-吡啶酚（TCP）污染土壤的修复效果.结果表明，菌株可以进一步降解2-HP，依次生成尼古丁蓝、马来酰胺酸和反丁烯二酸，直至转化成菌株DT-1生长的碳源.接种菌株DT-1对污染土壤中TCP的降解起到较大的促进作用，2组试验土壤中TCP （50mg/kg）降解率分别为94.4%和86.7%，未接种菌株的土壤中TCP降解率仅为20.4%和28.4%.带有绿色荧光蛋白基因gfp标记的菌株DT-1-gfp可在土壤中存活35d以上，并对TCP污染土壤的细菌群落丰度有显著的恢复作用.

Biodegradation of 3,5,6-trichloro-2-pyridinol by Cupriavidus sp. DT-1 in liquid and soil environments

Cupriavidus sp. DT-1 was a 3,5,6-trichloro-2-pyridinol (TCP)-degrading strain which could transform TCP to 2-hydroxypyridine (2-HP). Liquid-mass spectrometry (HPLC-MS) was used to detect the degradation products of 2-HP, And the methods of triparetal conjugation, quantitative real-time PCR (q-PCR) were used to evaluate the remediation effect of TCP-contaminated soils by the degrading-bacterium. Results showed that strain DT-1 was able to further degrade 2-HP, and sequentially produced nicotine blue, maleamic acid and fumaric acid, until it was transformed into the carbon source that could support the growth of strain DT-1. Pilot experiment showed that inoculation of strain DT-1 remarkably accelerated the elimination of TCP in soils. The degradation rates of TCP in inoculated soils were 94.4% and 86.7%, while those in uninoculated soils were 20.4% and 28.4%, respectively. Green fluorescent protein encoding gene gfp harbored strain DT-1-gfp could survive in soils for more than 35d. The results of q-PCR showed that inoculation of strain DT-1-gfp significantly improved the recovery of bacterial community abundance in the TCP-contaminated soils.