垃圾填埋场渗滤液诱发小鼠骨髓细胞微核效应

研究了垃圾填埋场渗滤液诱发小鼠骨髓嗜多染红细胞(PCE)微核(MN)效应.结果表明,渗滤液可诱发微核的形成,且呈明确的浓度(渗滤液COD_Cr)-效应关系.在亚急性染毒条件下,渗滤液COD_Cr为10mg/L时,可诱发微核率和微核细胞率显著高于阴性对照(n=16,P<0.001);在亚慢性染毒条件下,渗滤液COD_Cr为5mg/L时,即可诱发微核率和微核细胞率显著高于阴性对照(n=16,P<0.001).这一结果从整体水平上证明了垃圾填埋场渗滤液对哺乳动物具有遗传损伤效应,同时提示可以以小鼠骨髓嗜多染红细胞微核试验结果作为渗滤液生物监测指标.渗滤液处理时间越长,引起细胞遗传损伤所需的最低浓度越低,意味着长期接触环境低浓度渗滤液污染有引起体内细胞遗传物质损伤的潜在危险.

Micronuclei effect induced by refuse landfill leachate in mouse bone marrow cell

Micronuclei (MN) effect induced in the polychromatophilic erythroblast (PCE) of mouse bone marrow by refuse landfill leachate was studied. Leachate could induce formation of micronuclei, and present definite concentration (leachate CODCr)-effect relation. Under subacute toxic condition, leachate CODCr of 10mg/L could induce the rates of micronuclei and its cell higher markedly than the negative contract (n=16, P<0.001), and under subchronic condition, CODCr of 5mg/L could induce just the same. The leachate possessed genetic damage effect on mammal, and indicated meanwhile that the experiment results of PCE could be used possibly as leachate for biological detection index; and that the lowest concentration of leachate to induce the genetic damage was lower with longer treating time of leachate, meaning that long-exposing to leachate pollution at low concentration in environment might induce a potential risk of damage of internal cytogenetic material of human beings.