| 辽河口芦苇湿地细菌和古菌群落周期日变化特征 |
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| 引用本文： | 李明月,米铁柱,甄毓,王勋功.辽河口芦苇湿地细菌和古菌群落周期日变化特征[J].中国环境科学,2019,39(2):849-858. |
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| 作者姓名： | 李明月 米铁柱 甄毓 王勋功 |
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| 作者单位： | 1. 青岛海洋科学技术国家实验室海洋生态与环境科学功能实验室, 山东 青岛 266071;
2. 海洋环境与生态教育部重点实验室, 山东 青岛 266100;
3. 中国海洋大学环境科学与工程学院, 山东 青岛 266100 |
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| 基金项目： | 国家重点研发计划课题（2017YFC1404402）；鳌山科技创新计划项目（2016ASKJ02）；国家自然科学基金资助项目（41620104001） |
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| 摘 要： | 采用实时荧光定量PCR和Illumina高通量测序技术对秋季辽河口芦苇湿地水体中一昼夜细菌和古菌群落特征进行了研究.测序共获得52802条细菌和106091条古菌高质量序列，以97%的相似性聚类并抽平后共划分了530和979个OTU.结果表明，湿地水体中浮游细菌和古菌群落丰度均在早上达到最高值，夜晚达到最低值.细菌和古菌群落多样性指数均存在周期日变化，不同时间细菌和古菌群落结构和组成具有显著差异.此外，古菌群落组成特征表明辽河口芦苇湿地具有较高的潜在甲烷生成能力.本研究为进一步认识河口湿地细菌和古菌群落特征和功能奠定了基础.
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| 关 键 词： | 湿地 16S rRNA 细菌 古菌 群落结构 |
| 收稿时间： | 2018-06-21 |
Diurnal variations of bacterial and archaeal communities in the reed wetland of Liaohe estuary |
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| LI Ming-yue,MI Tie-zhu,ZHEN Yu,WANG Xun-gong.Diurnal variations of bacterial and archaeal communities in the reed wetland of Liaohe estuary[J].China Environmental Science,2019,39(2):849-858. |
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| Authors: | LI Ming-yue MI Tie-zhu ZHEN Yu WANG Xun-gong |
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| Institution: | 1. Laboratory for Marine Ecology and Environmental Science, Qingdao National Laboratory for Marine Science and Technology, Qingdao 266071, China;
2. Key Laboratory of Marine Environment and Ecology, Ministry of Education, Ocean University of China, Qingdao 266100, China;
3. College of Environmental Science and Engineering, Ocean University of China, Qingdao 266100, China |
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| Abstract: | The present study investigated the diurnal variations of bacterial and archaeal communities in Liaohe estuarine wetland in autumn, using the real-time fluorescence quantitative PCR method and Illumina high-throughput sequencing. The results showed that 52802 bacterial and 106091 archaeal sequences were obtained, which could be classified into 530 and 979 OTUs in 97% similarity level, respectively. The results showed that bacterial and archaeal 16S rRNA gene abundances both had the highest values in the morning, and the lowest ones at midnight. There were diurnal variations in the bacterial and archaeal community α-diversity indices, and obvious temporal changes were also observed in the bacterial and archaeal community compositions and structures. In addition, Liaohe estuarine wetland had high potential ability of methanogenesis which could be reflected by the composition of archaeal community. This study provides the basic information and knowledge to further understand the characteristics and functions of bacterial and archaeal communities in estuarine wetlands. |
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| Keywords: | wetland 16S rRNA bacteria archaea community structure |
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