DINP皮肤暴露对小鼠过敏性皮肤炎症的佐剂作用

为了探究新型增塑剂邻苯二甲酸二异壬醋(DINP)对小鼠过敏性皮肤炎症的影响,采用皮肤染毒的暴露方式,56只SPF级雄性Balb/C小鼠随机平均分为7组处理40d用0、1.4、14和140mg/kg DINP对小鼠进行皮肤染毒,并用抗氧化剂褪黑素设置2组来探究氧化应激的介导作用,以及1组生理盐水对照组染毒结束后取耳组织测定耳肿指数和双耳重量差,并制作耳组织病理学切片,最后检测氧化应激指标.实验结果显示,14和140mg/kg DINP皮肤暴露组的皮肤炎症有明显加剧(p0.01),氧化应激指标也有明显变化(p0.01).这表明,一定浓度的DINP,经较长时间的暴露,对小鼠过敏性皮肤炎症具有明显的佐剂作用、并且,随着DINP浓度升高,小鼠机体氧化应激过度活化,造成机体组织或细胞的氧化损伤,并加剧了炎症反应.

Adjuvant effect of skin DINP exposure on mice with allergic dermatitis

In order to explore the effect of a new plasticizer-di-isononyl phthalate (DINP) on mice allergic skin inflammation, we used skin exposure method. Fifty six male Balb/C mice were divided into seven groups, in which four groups and exposed to 0, 1.4, 14 and 140mg/kg DINP, two other groups administrated antioxidant melatonin were set to explore oxidative stress effects, and one group was set as saline control. After exposure, the indexes of ear swelling and the weight difference of bilateral ear were determined, and we observed the pathology change through the ear pathology slice. The biomarkers about oxidative stress were tested too. The results showed that the 14and 140mg/kg DINP worsen skin inflammation significantly (P<0.01), and the oxidative stress indexes also changed obviously (P<0.01). These results suggest that a certain concentration of DINP can significantly increase related symptom of skin inflammation caused by allergen. It certifies that a certain concentration DINP has obvious adjuvant effect on allergic skin inflammation in mice. In addition, along with the concentration of DINP increase, the oxidative stress in the bodies of mice is overactive and it causes organism tissues or cells oxidative damage and contributes to the inflammatory response.