草甘膦对同源假单胞基因工程菌代谢活性的影响

基于微量热法,研究两株基因缺失型斯氏假单胞杆菌对草甘膦的耐受毒性和降解作用,结果表明:草甘膦可以作为菌株的能量来源;草甘膦对P.stutzeri WM 581和P.stutzeri WM 567的半抑制浓度分别为47.47,43.26mg/L.两菌株对草甘膦均有较快的降解速率,其降解的半衰期约为9~17h,而草甘膦作为2类菌株生长过程中可以代谢的营养物质,其作为唯一碳源时的降解率(64%)要高于作为磷源时的降解率(43%).pH值与盐度对两菌株的生长有较大影响,草甘膦降解的较佳条件为:pH值为6,盐度低于0.5g/L.

Metabolic activity effects of glyphosate on homologous gene engineering bacteria of Pseudomonas

On the basis of microcalorimetric technology, we investigated the effect of glyphosate on metabolic activity of two homologous gene engineering bacteria of Pseudomonas. Glyphosate can serve as nutrients of these two strains.The half inhibitory concentration of glyphosate for P.stutzeri WM 581 and P.stutzeri WM 567 were 47.47mg/L and 43.26mg/L respectively. Both of strains can degrade glyphosate rapidly, and the half-life was approximate 9~17 hours. And as metabolism nutrients of these two strains, the degradation rate of glyphosate as carbon source (64%) is higher than as phosphorus source (43%). Salinity and pH impacted the growth of these two strains remarkably, the relatively optimal condition to degrade glyphosate is: pH = 6, NaCl < 0.5g/L.