离子液体1-丁基-3-甲基咪唑氯盐对斜生栅藻的毒性效应

按照毒性试验标准方法研究了不同浓度离子液体1-丁基-3-甲基咪唑氯盐(BMIM]Cl)对斜生栅藻的影响,测定了半数抑制浓度(IC50)、叶绿素含量、超氧化物歧化酶(SOD)活性、过氧化氢酶(CAT)活性以及藻细胞通透性的变化,观察了80mg/LBMIM]Cl处理96h对藻细胞的影响.结果表明:BMIM]Cl对斜生栅藻生长具有抑制作用,随着浓度的增大抑制率增加,叶绿素含量下降, 48h IC50、72h IC50和96h IC50分别为103.77、76.44和68.49mg/L.藻细胞对BMIM]Cl有一定的氧化应激反应,CAT和SOD活性随BMIM]Cl浓度升高而降低.BMIM]Cl对藻细胞的细胞膜有一定程度的破坏作用,藻细胞通透性随浓度升高而增大,同时藻细胞的超微结构受到了BMIM]Cl的影响,造成质壁分离,叶绿体片层断裂,线粒体嵴数量减少.

Toxicity of 1-butyl-3-methylimidazolium chloride ionic liquid to Scenedesmus obliqnus

The toxicity of ionic liquid 1-butyl-3-methylimidazolium chloride(Cl) to Scenedesmus obliqnus was studied in different concentration by using the standard toxicity test.Five different test endpoints were determined such as the growth inhibition rate(IC50),the content of chlorophyll a(Chla),chlorophyll b(Chlb),the superoxide dismutase activity(SOD) and the catalase activity(CAT),the permeability of algae cell,and the ultrastructural morphology of cell.The results indicated that the inhibition rate of algae growth increased and the content of Chla and Chlb in algae cell decreased with the Cl concentration.The 48h IC50,72h IC50 and 96h IC50 were 103.77、76.44 and 68.49mg/L,respectively.The results also indicated that the activities of CAT and SOD decreased due to the oxidative stress and the permeability of algal cell decreased due to the cell membrane being destroyed with the increase of Cl concentration.Also,the ultrastructure of algal cell was affected by 80mg/L Cl,resulting in plasmolysis,chloroplast lamellae fractured and the number of crest in mitochondria reduced.