聚糖菌颗粒污泥的有机物吸收及胞内储存特性

采用特殊运行方式的厌氧-好氧SBR系统(厌氧后排水),在乙酸钠、葡萄糖及葡萄糖-乙酸钠混合基质条件下均培养出了稳定的聚糖菌颗粒污泥.通过对典型周期有机物、磷酸盐、胞内糖原及聚β-羟基丁酸(PHB)变化的测定分析,证明有机基质的种类对于聚糖菌能量利用模式、有机物吸收速率及胞内储存物质种类具有显著的影响.污泥初始胞内糖原水平是有机物吸收数量及吸收速率的关键影响因素,当糖原水平低于0.05g/gSS时,厌氧有机物去除率与糖原水平直接相关:而糖原水平高于0.05g/gSS时,厌氧有机物去除率趋于稳定.不同糖原水平污泥厌氧吸收有机物的速率具有明显差异,在厌氧初期有机物快速吸收阶段,有机物厌氧吸收速率随胞内糖原水平升高而增加.

Characters of organics uptake and intracellular polymer storage of GAOs granular sludge

Stable glycogen accumulating organisms (GAOs) granules were cultivated in three sequencing batchreactors(SBR), fed with acetate, glucose and glucose-acetate as organic substrate respectively, by means of speciaalternating anaerobic-aerobic operation mode, i.e. withdrawal after anaerobic period and sludge aeration during aerobicperiod to suppress the activity of phosphate accumulating organisms(PAOs). Analyzing the variation of organicsphosphate, intracellular glycogen and PHB in typical cycle verified that the sort of organic substrate had marked influenceon the energy utilization mode, organics uptake rate and intracellular storage polymer sort of GAOs. Moreover, the initiaintracellular glycogen content was the key factor influencing the organics uptake amount and uptake rate. whenintracellular glycogen content was below 0.05g/g SS, anaerobic organics uptake efficiencies appeared to be dependent onglycogen content, and organics uptake efficiencies tended to be stable as glycogen content was higher than 0.05g/g SS. Inaddition, anaerobic organics uptake rate was in great difference with different glycogen content, during fast uptake periodin initial anaerobic stage, anaerobic organics uptake rate was increased with intracellular glycogen content increased.