亚慢性全氟辛烷磺酸染毒对大鼠海马细胞钙稳态的影响

采用经口摄食染毒,探讨全氟辛烷磺酸(PFOS)对大鼠血清与脑组织中PFOS浓度及海马细胞内钙离子浓度(Ca2+]i)的影响,观察大鼠大脑皮质、海马和小脑组织病理学变化.结果表明,染毒组大鼠血清与脑组织中PFOS浓度、海马细胞内Ca2+]i均显著高于对照组,且随着染毒剂量的升高,海马细胞内Ca2+]i升高.大鼠血清与脑组织中PFOS浓度、脑组织中PFOS浓度与海马细胞Ca2+]i均呈现显著的正相关关系.染毒组大鼠大脑皮质、海马和小脑组织中未见尼氏体染色变浅等病理性改变.

Effect of perfluorooctane sulfonate on intracellular free calcium level in rats' hippocampus

Effect of subchronic perfluorooctane sulfonate (PFOS) exposure on free calcium level (Ca~(2+)]_i) in hippocampus in rats and the mechanism of the neurotoxicity induced by PFOS was studied. The adult male Wistar rats were treated with oral PFOS exposure at dosage of 2, 8, 32, 128mg/kg, respectively. After 60 days' exposure, the rats were sacrificed, and then single-cell suspension of hippocampus was prepared. Ca~(2+)]_i was determined with Fura-2/AM fluorescence probe. The brain and serum were concentrated by liquid-phase extraction and then the concentration of PFOS was determined using HPLC/MS. The cortex, hippocampus and cerebellum were collected for evaluation of histopathology change. PFOS concentration in serum and brain in PFOS treated groups was significantly higher than that in the control group (P<0.01). There was a significant positive correlation between Ca~(2+)]_i and PFOS concentration in serum and brain. No obvious change was observed in the morphology of the brain in rats exposed to PFOS.