东方伊萨酵母YP-1对染料活性艳红K-2BP的脱色

利用含染料的选择性培养基从土壤中分离出一株对活性艳红K-2BP有明显脱色效果的酵母菌株YP-1,经鉴定为东方伊萨酵母Issatchenkia orientalis.结果表明,该酵母菌对￡400mg/L的活性艳红K-2BP有较好的脱色效果.对于活性艳红K-2BP起始浓度为100mg/L的培养基,该菌株可在12h达到99%以上的最大脱色率,其最佳接种量为10%(体积分数),最适pH值在3~9之间,氮源(NH4)2SO4的浓度30.02%,碳源葡萄糖的浓度30.2%.脱色机理研究结果表明,该酵母对活性艳红K-2BP的去除是先吸附后生物降解.此外,该菌株对初始浓度为200mg/L的偶氮染料活性黑KN-B的脱色率也可达99.5%.

Decolorization of azo dye reactive brilliant red K-2BP by a novel yeast Issatchenkia orientalis YP-1

An yeast strain YP-1 was isolated on selective media containing azo dye reactive brilliant red K-2BP, and identified as Issatchenkia orientalis. The strain YP-1 could effectively decolorize reactive brilliant red K-2BP within the concentration of 400mg/L. It gave a maximal decolorization of 99% for reactive brilliant red K-2BP of 100mg/L after incubation for 12h, under the optimal culture conditions: inoculation volume 10%(V/V), pH within the range from 3 to 9, concentration of (NH4)2SO4 30.02%, and concentration of glucose 30.2%. The strain YP-1 could absorb reactive brilliant red K-2BP and subsequently biodegrade it. The strain YP-1 could also decolorize another azo dye reactive black 5 of 200mg/L, up to the decolorization of 99.5%.