利用磷脂脂肪酸表征人工湿地微生物群落结构

通过磷脂脂肪酸(PLFAs)分析,研究了复合垂直流人工湿地基质剖面的微生物群落结构及其分布特征.结果表明,湿地PLFAs组成以饱和脂肪酸、支链脂肪酸和单不饱和脂肪酸为主,多不饱和脂肪酸与环丙烷脂肪酸含量较少.特征脂肪酸的比值呈垂直分布.基质中好氧原核微生物为优势类群,其次为革兰氏阳性细菌及其他厌氧细菌,真核微生物所占比例最低.统计结果显示,好氧原核微生物的特征PLFAs相对含量在上行池表层最高;革兰氏阳性细菌、SRB及其他厌氧细菌的特征PLFAs相对含量在下行池中层显著高于其他位点,指示该区域为湿地系统主要的兼性厌氧功能区.

Microbial community structure in the integrated vertical-flow constructed wetland utilizing phospholipid fatty acids analysis

The microbial community structure and its distribution characteristics of the substrate section in the integrated vertical-flow constructed wetland were studied through phospholipid fatty acids(PLFAs)analysis.The composition of wetland PLFAs was mainly the saturated fatty acid,branched fatty acid and monounsaturated fatty acid;the contents of polyunsaturated fatty acid and cyclopropane fatty acid were relatively small in amount.The ratios of characteristic fatty acid submitted vertical distribution pattern.The aerobic prokaryotes in substrates were predominant groups,next were the gram-positive bacteria and other anaerobic bacteria;and micro eukaryotes occupied the lowest proportion.The statistical results showed that the relative contents of aerobic prokaryotes was highest in the surface layer of the up-flow chamber,while the characteristic PLFAs relative contents of gram-positive bacteria,sulfate reducing bacteria(SRB)and other anaerobic bacteria were highest in the middle layer of the down-flow chamber,suggesting that this area was the main facultative anaerobic function area in the wetland system.