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鞘氨醇单胞菌USTB-05对微囊藻毒素的生物降解
引用本文:徐慧敏,闫,海,马,松,王华生,尹春华,刘晓璐.鞘氨醇单胞菌USTB-05对微囊藻毒素的生物降解[J].中国环境科学,2014,34(5):1316-1321.
作者姓名:徐慧敏          王华生  尹春华  刘晓璐
作者单位:北京科技大学化学与生物工程学院;江西理工大学建筑与测绘工程学院;
基金项目:国家自然科学基金资助项目(21177009,11071013);教育部博士点基金资助项目(20120006110001)
摘    要:研究了云南滇池水华蓝藻细胞中微囊藻毒素(MCs)的分析和鞘氨醇单胞菌USTB-05在细胞水平和酶水平下对MC-YR、RR和LR的生物降解.结果表明,云南滇池水华蓝藻细胞中MC-YR、RR和LR的含量分别为0.16, 0.96, 0.47mg/g.在初始浓度分别为19.5mg/L MC-YR、79.5mg/L MC-RR和43.6mg/L MC-LR下,鞘氨醇单胞菌USTB-05在2d内可将上述3种MCs全部降解,鞘氨醇单胞菌USTB-05粗酶液可以以更快的速率对MC-YR、MC-RR和MC-LR进行高效酶催化降解,在10h内可以将初始浓度分别为14.8mg/L MC-YR、28.4mg/L MC-RR和19.5mg/L MC-LR全部降解.同时发现了MC-YR降解过程中的2个中间和1个最终代谢产物.

关 键 词:微囊藻毒素  鞘氨醇单胞菌USTB-05    生物降解  

Biodegradation of microcystins by Sphingopyxis sp
XU Hui-Min,YAN Hai,MA Song,WANG Hua-Sheng,YIN Chun-Hua,LIU Xiao-Lu.Biodegradation of microcystins by Sphingopyxis sp[J].China Environmental Science,2014,34(5):1316-1321.
Authors:XU Hui-Min  YAN Hai  MA Song  WANG Hua-Sheng  YIN Chun-Hua  LIU Xiao-Lu
Abstract:The contents of microcystins (MCs) in dry cyanobacterial cells taken from Dianchi Lake and the biodegradation of MC-YR, RR and LR by Sphingopyxis sp. USTB-05 at the cellular and enzyme levels were studied. The contents of MC-YR, RR and LR in dry cyanobacterial cells were 0.16, 0.96 and 0.47mg/g, respectively. Initial concentrations of 19.5mg/L MC-YR, 79.5mg/L MC-RR and 43.6mg/L MC-LR were completely biodegraded by Sphingopyxis sp. USTB-05 within 2d. Further studies indicated that MC-YR, RR and LR could also be biodegraded in more rapid rates by crude enzymes of Sphingopyxis sp. USTB-05, and initial MC-YR, RR and LR of 14.8, 28.4, 19.5mg/L were completely removed within 10h, respectively, and two intermediate and one final products were found during the biodegradation process of MC-YR catalyzed by the crude enzymes of USTB-05.
Keywords:microcystins  Sphingopyxis sp  USTB-05  enzyme  biodegradation  
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