菖蒲对铜绿微囊藻的化感作用

 采用两厢培养池研究了菖蒲(Acoruscalamus)对铜绿微囊藻(Microcystisaeruginosa)的化感抑制作用.结果表明,在排除藻菌作用和营养竞争前提下,共培养条件下的菖蒲可抑制微囊藻的生长,使藻液光密度(OD₆₈₀)降低;抑制效应取决于菖蒲和微囊藻之间的相对生物量,实验条件下100g菖蒲在初始藻液光密度为0.2时有最强抑藻效应;抑藻化感物质的释放与菖蒲根茎密切相关,根茎受损的菖蒲无抑藻效应;在抑藻过程中对藻细胞的生理指标分析表明,菖蒲的存在降低了藻细胞内的蛋白含量,使SOD、MDA和CAT等抗氧化系统酶的活性增加,化感物质造成活性氧的过量积累可能是微囊藻死亡的原因之一.

Allelopathic effect of Acorus calamus on Microcystis aeruginosa

Inhibitory effects of Acorus calamus on Microcystis aeruginosa were studied under coexistence conditions. Through cultured in two-cell container and excluded the influence of bacteria and nutrient, A. calamus could inhibit the growth of M. aeruginosa, which was observed by the decrease in optical density (OD680) of the culture. The intensity of allelopathy was related to the comparatively biomass of A. calamus and M. aeruginosa, for 100g of A. calamu demonstrated the strongest inhibitory effect at the original OD680 of 0.2 in the experiment. The allelochemics could be secreted from the roots of A. calamus, and A. calamus with injured roots showed no inhibitory effect. The physiological analysis of alga cell in the process showed that the existence of A. calamus lowered the content of proteins in cell, causing the antioxidative enzyme activities including SOD, MDA and CAT increase. The inhibitory effects might due to the excessive accumulation of reactive oxygen species.