常温ANAMMOX工艺运行性能及功能菌研究

通过改变进水基质浓度,对以火山岩为填料的常温上向流厌氧氨氧化生物滤池在不同基质浓度下的脱氮性能进行了研究.借助显微镜、电镜(SEM)观察对滤池内的微生物形态进行了分析,利用变性凝胶电泳(DGGE)和克隆测序等微生物方法,对ANAMMOX种属进行了鉴定.试验结果表明,常温条件下,厌氧氨氧化滤池在高、低不同基质下都能够保持较高的脱氮效果,其中TN去除负荷最高能够达到2.99kgN/(m3×d),NH4+-N和NO2--N最高去除率分别能够达到99.4%和98.8%;显微镜、电镜观察显示:滤池下部的微生物种类更丰富,ANAMMOX菌在滤池的中部和上部的比例更高;16SrRNA克隆测序鉴定结果表明:滤池中的ANAMMOX菌为Candidatus Kuenenia stuttgartiensis,其对温度和基质浓度都有着较宽的适应性.

Study on performance of ANAMMOX reactor and the functional bacteria at room temperature

At room tempertature, by changing the substrate concentration of inflow, the biological nitrogen removal of up-flowing ANAMMOX biofilter loading volcanic rock filter at different ammonium concentrations was investigated. techniques of microscope and scanning electron microscopy (SEM) were utilized to analyze microorganisms form, denaturing gradient gel electrophoresis (DGGE), cloning and sequcening were used to identify species of ANAMMOX. The results of operation revealed that ,at room temperature, the up-flow ANAMMOX biofilter had the ability to maintain high capacity of biological nitrogen removal under high and low substrate concentrations. The maximum total nitrogen removal load was up to 2.99kgN/(m3×d), and the maximum removal rate of NH4+-N and NO2--N was 99.4% and 98.8% respectively. Microscope and SEM show that the species of microorganisms is much richer at the bottom of biofilter, however, the proportion of ANAMMOX is much higher in the middle and upper of biofilter. The sequence of ANAMMOX 16SrRNA identification revealed that Candidatus Kuenenia stuttgartiensis occurred in the up–flowing ANAMMOX biofilter, which had wide adaptability to different temperature and substrate concentrations.